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Literature summary for 5.3.99.3 extracted from

  • Ogino, N.; Miyamoto, T.; Yamamoto, S.; Hayaishi, O.
    Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme (1977), J. Biol. Chem., 252, 890-895.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GSH dependent on Bos taurus

General Stability

General Stability Organism
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Bis(3-carboxy-4-nitrophenyl)disulfide
-
Bos taurus
HgCl2
-
Bos taurus
iodoacetamide
-
Bos taurus
iodoacetic acid
-
Bos taurus
Iodosobenzoic acid
-
Bos taurus
NEM
-
Bos taurus
PCMB
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
Prostaglandin H1
-
Bos taurus
0.05
-
Prostaglandin G1
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Bos taurus
-
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
prostaglandin H1 Bos taurus
1.8
-
prostaglandin H2 Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks Bos taurus
-70°C, 1 mM glutathione, pH 7.0, stable for at least several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin G1 at 50% of the activity relative to prostaglandin H Bos taurus 15-Hydroperoxy-prostaglandin E1 major product ?
prostaglandin G2 at 50% of the activity relative to prostaglandin H Bos taurus 15-hydroperoxy-prostaglandin E2 major product ?
Prostaglandin H1 ir Bos taurus Prostaglandin E1
-
?
Prostaglandin H1 i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-13-enoic acid Bos taurus Prostaglandin E1
-
?
Prostaglandin H2 i.e. 15-hydroxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid Bos taurus Prostaglandin E2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation Bos taurus
25
-
pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 almost inactive below pH 5.5 and above pH 9.5 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione Bos taurus