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Literature summary for 5.3.99.2 extracted from

  • Zhou, Y.; Shaw, N.; Li, Y.; Zhao, Y.; Zhang, R.; Liu, Z.J.
    Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules (2010), FASEB J., 24, 4668-4677.
    View publication on PubMed
Search for more literature for 5.3.99.2

Cloned(Commentary)

Cloned (Comment) Organism
mutant C65A is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant C65A in complex with oleic acid and palmitoleic acid, hanging drop vapor diffusion method, using 0.1 M citric acid (pH 4.0) and 1.7 M ammonium sulfate Homo sapiens

Protein Variants

Protein Variants Comment Organism
C65A inactive Homo sapiens
F83A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
K59A the mutation enhances the catalytic efficiency by more than 2fold Homo sapiens
L131A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
L79A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
M64A the mutation reduces the catalytic efficiency by almost 10fold Homo sapiens
M94A the mutation reduces the catalytic efficiency Homo sapiens
S45A the mutation reduces the catalytic efficiency Homo sapiens
S81A the mutation reduces the catalytic efficiency Homo sapiens
T147A the mutation slightly enhances the catalytic efficiency Homo sapiens
W54A the mutation reduces the catalytic efficiency Homo sapiens
Y149A the mutation enhances the catalytic efficiency by more than 2fold Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0102
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0123
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0127
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0138
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0154
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0161
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0202
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0204
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0245
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0252
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0325
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.0328
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P41222
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and Superdex G75 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
 Homo sapiens Prostaglandin D2
-
 ir

Subunits

Subunits Comment Organism
monomer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
L-PGDS
-
 Homo sapiens
L-prostaglandin D synthase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.63
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.81
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
0.87
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2.23
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2.24
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2.4
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2.64
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2.91
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
3.67
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.73
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
5.84
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
110
-
 prostaglandin H2 mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
1170
-
 prostaglandin H2 mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
1890
-
 prostaglandin H2 mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
2120
-
 prostaglandin H2 mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
6570
-
 prostaglandin H2 mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
6660
-
 prostaglandin H2 mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
8940
-
 prostaglandin H2 mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
9420
-
 prostaglandin H2 mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
11490
-
 prostaglandin H2 wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
14290
-
 prostaglandin H2 mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
24840
-
 prostaglandin H2 mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens
27050
-
 prostaglandin H2 mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C Homo sapiens