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Literature summary for 5.3.99.2 extracted from

  • Takahashi, S.; Tsurumura, T.; Aritake, K.; Furubayashi, N.; Sato, M.; Yamanaka, M.; Hirota, E.; Sano, S.; Kobayashi, T.; Tanaka, T.; Inaka, K.; Tanaka, H.; Urade, Y.
    High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors (2010), Acta Crystallogr. Sect. F, 66, 846-850.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
H-PGDS is crystallized in a microgravity environment at 20°C, using 30% (w/v) PEG 6000, 10 mM dithiothreitol, 10 mM glutathione, 1% (v/v) dioxane and 1 mM magnesium chloride in 50 mM Tris-HCl pH 8.4 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
HQL-79 i.e. 4-benzhydryloxy-1-[3-(1H-tetrazol-5-yl)-propyl]-piperidine, H-PGDS-specific inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Prostaglandin H2 Homo sapiens
-
Prostaglandin D2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography and Mono-Q column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mast cell
-
Homo sapiens
-
Th2-cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
Homo sapiens Prostaglandin D2
-
?

Synonyms

Synonyms Comment Organism
H-PGDS
-
Homo sapiens
haematopoietic PGD synthase
-
Homo sapiens
haematopoietic prostaglandin D synthase
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0059
-
pH and temperature not specified in the publication Homo sapiens HQL-79