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Literature summary for 5.3.99.2 extracted from

  • Uchida, Y.; Urade, Y.; Mori, S.; Kohzuma, T.
    UV resonance Raman studies on the activation mechanism of human hematopoietic prostaglandin D(2) synthase by a divalent cation, Mg(2+) (2010), J. Inorg. Biochem., 104, 331-340.
    View publication on PubMed
Search for more literature for 5.3.99.2

Activating Compound

Activating Compound Comment Organism Structure
Mg2+
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pT7-7 for expression in Escherichia coli BL21DE3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y8F active site mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 monomer Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Prostaglandin H2 Homo sapiens
-
 Prostaglandin D2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60760
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation, and on a GSH Sepharose 4B and a Superdex 75 pg column Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
 Homo sapiens Prostaglandin D2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 23000 Da Homo sapiens

Synonyms

Synonyms Comment Organism
H-PGDS
-
 Homo sapiens
hematopoietic prostaglandin D2 synthase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 kinetic assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 kinetic assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
glutathione
-
 Homo sapiens