Literature summary for 5.3.99.2 extracted from

Pinzar, E.; Miyano, M.; Kanaoka, Y.; Urade, Y.; Hayaishi, O.
Structural basis of hematopoietic prostaglandin D synthase activity elucidated by site-directed mutagenesis (2000), J. Biol. Chem., 275, 31239-31244.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
C156L	loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity	Rattus norvegicus
C156Y	loss of prostaglandin D synthase activity, retention of glutathione S-transferase activity	Rattus norvegicus
K112E	retention of prostaglandin D synthase and glutathione S-transferase activity	Rattus norvegicus
K198E	retention of prostaglandin D synthase and glutathione S-transferase activity	Rattus norvegicus
L199F	retention of prostaglandin D synthase and glutathione S-transferase activity	Rattus norvegicus
R14E	complete loss of prostaglandin D synthase activity and glutathione S-transferase activity	Rattus norvegicus
R14K	complete loss of prostaglandin D synthase activity and glutathione S-transferase activity	Rattus norvegicus
W104I	complete loss of prostaglandin D synthase activity and glutathione S-transferase activity	Rattus norvegicus
Y152F	retention of prostaglandin D synthase and glutathione S-transferase activity	Rattus norvegicus
Y8F	complete loss of prostaglandin D synthase activity and glutathione S-transferase activity	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	glutathione	-	Rattus norvegicus
0.5	-	(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	-	Rattus norvegicus
0.5	-	glutathione	conjugation of glutathione to 1-chloro-2,4-dinitrobenzene	Rattus norvegicus
3	-	1-chloro-2,4-dinitrobenzene	conjugation of glutathione to 1-chloro-2,4-dinitrobenzene	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH	Rattus norvegicus	-	(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	O35543	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
30	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	-	Rattus norvegicus	(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate	-	?
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + 2 GSH	-	Rattus norvegicus	(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + GSSG + 2 H+	-	?
glutathione + 1-chloro-2,4-dinitrobenzene	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
Hematopoietic prostaglandin D synthase	i.e. PGDS	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	-	glutathione	conjugation of glutathione to 1-chloro-2,4-dinitrobenzene	Rattus norvegicus
5	-	1-chloro-2,4-dinitrobenzene	conjugation of glutathione to 1-chloro-2,4-dinitrobenzene	Rattus norvegicus
15	-	glutathione	-	Rattus norvegicus
21.7	-	(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	-	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
glutathione	-	Rattus norvegicus

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Release 2023.1 (January 2023)