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Literature summary for 5.3.4.1 extracted from

  • Abromaitis, S.; Stephens, R.S.
    Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase (2009), PLoS Pathog., 5, e1000357.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzymatically nonfunctional PDI can restore Chlamydia sp. attachment but not entry into mutant CHO6 cells, while expression of functional PDI restores the complete Chlamydia infection, overview Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
additional information a naturally occuring mutant strain with mutated, nonfunctional PDI shows complete resistance to infection, attachement and entry, of Chlamydia species, expression of enzymatically nonfunctional PDI can restore Chlamydia sp. attachment but not entry into mutant CHO6 cells, while expression of functional PDI restores the complete Chlamydia infection, overview Cricetulus griseus
additional information HeLa cells are transfected with PDI-targeting siRNA for PDI knockdown leading to resistance to Chlamydia inefctions Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
bacitracin a PDI-specific inhibitor Cricetulus griseus
bacitracin a PDI-specific inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Cricetulus griseus 9986
-
cell surface
-
 Homo sapiens 9986
-
membrane
-
 Cricetulus griseus 16020
-
membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cricetulus griseus the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview ?
-
 ?
additional information Homo sapiens the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO-K1 cell
-
 Cricetulus griseus
-
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview Cricetulus griseus ?
-
 ?
additional information the PDI protein is necessary for Chlamydia attachment, but the bacteria apparently do not bind directly to cell-associated PDI, suggesting that Chlamydia attaches to a host protein(s) associated with PDI. PDI enzymatic activity is necessary for bacterial entry but not for attachment, cell surface PDI-mediated reduction triggers Chlamydia entry into cells, molecular mechanism, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PDI
-
 Cricetulus griseus
PDI
-
 Homo sapiens
protein disulfide isomerase
-
 Cricetulus griseus
protein disulfide isomerase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cricetulus griseus
37
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme is required for infection by Chlamydia species Homo sapiens