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Literature summary for 5.3.4.1 extracted from

  • Gruber, C.W.; Cemazar, M.; Clark, R.J.; Horibe, T.; Renda, R.F.; Anderson, M.A.; Craik, D.J.
    A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins (2007), J. Biol. Chem., 282, 20435-20446.
    View publication on PubMed
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
gene pdi, DNA and amino acid sequence determination and analysis, His-tagged PDI is co-expressed with the cyclotide precursor protein Oak1 in Escherichia coli strain BL21(DE3) using the pREP4 helper plasmid Oldenlandia affinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information folding kinetics of linear and cyclic kalata B1 Oldenlandia affinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, recombinant His-tagged enzyme, SDS-PAGE Oldenlandia affinis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oldenlandia affinis the enzyme is involved in the oxidative folding of cystine knot defense proteins and in in the biosynthesis of insecticidal cyclotides, overview, the Oldenlandia affinis plant accumulates knotted circular proteins called cyclotides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oldenlandia affinis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PDI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Oldenlandia affinis

Reaction

Reaction Comment Organism Reaction ID
catalyses the rearrangement of -S-S- bonds in proteins mechanism of enzyme-assisted folding of plant cyclotides Oldenlandia affinis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kalata B1 and derivatives, PDI dramatically enhanced the correct oxidative folding of linear and cyclic kalata B1 at physiological pH, determination of folding intermediates Oldenlandia affinis ?
-
 ?
additional information the enzyme is involved in the oxidative folding of cystine knot defense proteins and in in the biosynthesis of insecticidal cyclotides, overview, the Oldenlandia affinis plant accumulates knotted circular proteins called cyclotides Oldenlandia affinis ?
-
 ?
additional information PDI interacts with the cyclotide precursor protein Oak1, PDi is a functional oxidoreductase and exhibits both protein disulfide isomerase and chaperone activity, properties of disulfide species, overview Oldenlandia affinis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, recombinant His-tagged enzyme, SDS-PAGE Oldenlandia affinis
More primary and secondary PDi structure analysis, overview Oldenlandia affinis

Synonyms

Synonyms Comment Organism
PDI
-
 Oldenlandia affinis
protein-disulfide isomerase
-
 Oldenlandia affinis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Oldenlandia affinis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Oldenlandia affinis

Cofactor

Cofactor Comment Organism Structure
GSH for oxidative folding Oldenlandia affinis
GSSG for reduction activity Oldenlandia affinis

pI Value

Organism Comment pI Value Maximum pI Value
Oldenlandia affinis PDI C-terminal extension domain, isoelectric focusing
-
 6.6