Cloned (Comment) | Organism |
---|---|
cloning from RNA by 3' race RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparison, expression as GST-tagged or His-tagged enzyme in Escherichia coli strain BL21(DE3) | Amblyomma variegatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | PDI contains an endoplasmic reticulum-retention KEEL signal | Amblyomma variegatum | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Amblyomma variegatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Amblyomma variegatum | PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amblyomma variegatum | A0SHR2 | a tick cultured on rabbit hosts, constitutive gene | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged or His-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione or nickel affinity chromatography, respectively, native enzyme partially by isoelectric focusing/2D PAGE | Amblyomma variegatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibre tract | cells of the acini type II and III of salivary gland | Amblyomma variegatum | - |
gonad | male tick | Amblyomma variegatum | - |
additional information | the PDI expression levels in salivary gland is uniform in unfed ticks and ticks fed for different time intervals, no differences in enzyme expression between female and male ticks, PDI tissue distribution analysis, overview | Amblyomma variegatum | - |
salivary gland | - |
Amblyomma variegatum | - |
trachea | male ticks | Amblyomma variegatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
insulin + DTT | bovine substrate, reductase activity with DTT | Amblyomma variegatum | ? | - |
? | |
additional information | PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding | Amblyomma variegatum | ? | - |
? | |
additional information | the enzyme shows dithiol-disulfide-oxidoreductase activity, and contains a conserved WCGHC active site and two thioredoxin domains | Amblyomma variegatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Amblyomma variegatum |
More | domain organization of PDI, overview | Amblyomma variegatum |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the family of protein disulfide isomerases | Amblyomma variegatum |
PDI | - |
Amblyomma variegatum |
protein disulfide isomerase | - |
Amblyomma variegatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Amblyomma variegatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Amblyomma variegatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Amblyomma variegatum | isoelectric focusing | - |
6.64 |