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Literature summary for 5.3.4.1 extracted from

  • Knizetova, P.; Vancova, I.; Kocakova, P.; Slovak, M.; Proost, P.; Kopacek, J.
    New member of the protein disulfide isomerase (PDI) family identified in Amblyomma variegatum tick (2006), Insect Biochem. Mol. Biol., 36, 943-953.
    View publication on PubMed
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
cloning from RNA by 3' race RT-PCR, DNA and amino acid sequence determination and analysis, sequence comparison, expression as GST-tagged or His-tagged enzyme in Escherichia coli strain BL21(DE3) Amblyomma variegatum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum PDI contains an endoplasmic reticulum-retention KEEL signal Amblyomma variegatum 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Amblyomma variegatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Amblyomma variegatum PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding ?
-
 ?

Organism

Organism UniProt Comment Textmining
Amblyomma variegatum A0SHR2 a tick cultured on rabbit hosts, constitutive gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged or His-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione or nickel affinity chromatography, respectively, native enzyme partially by isoelectric focusing/2D PAGE Amblyomma variegatum

Source Tissue

Source Tissue Comment Organism Textmining
fibre tract cells of the acini type II and III of salivary gland Amblyomma variegatum
-
gonad male tick Amblyomma variegatum
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additional information the PDI expression levels in salivary gland is uniform in unfed ticks and ticks fed for different time intervals, no differences in enzyme expression between female and male ticks, PDI tissue distribution analysis, overview Amblyomma variegatum
-
salivary gland
-
 Amblyomma variegatum
-
trachea male ticks Amblyomma variegatum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insulin + DTT bovine substrate, reductase activity with DTT Amblyomma variegatum ?
-
 ?
additional information PDI has an important function in the correct folding of nascent polypeptides, which is a crucial step in the mechanism which delivers tick proteins to the secretion pathway important for blood feeding Amblyomma variegatum ?
-
 ?
additional information the enzyme shows dithiol-disulfide-oxidoreductase activity, and contains a conserved WCGHC active site and two thioredoxin domains Amblyomma variegatum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Amblyomma variegatum
More domain organization of PDI, overview Amblyomma variegatum

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the family of protein disulfide isomerases Amblyomma variegatum
PDI
-
 Amblyomma variegatum
protein disulfide isomerase
-
 Amblyomma variegatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Amblyomma variegatum

Cofactor

Cofactor Comment Organism Structure
DTT
-
 Amblyomma variegatum

pI Value

Organism Comment pI Value Maximum pI Value
Amblyomma variegatum isoelectric focusing
-
 6.64