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Literature summary for 5.3.4.1 extracted from
- The nonconsecutive disulfide bond of Escherichia coli phytase (AppA) renders it dependent on the protein-disulfide isomerase, DsbC (2005), J. Biol. Chem., 280, 11387-11394.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the mutational introduction of the AppA nonconsecutive disulfide bond into the AppA homologue Agp, which is independent of DsbC, renders the Agp mutant dependent on DsbC, overview | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Escherichia coli | - |
- |
| periplasm | - |
Escherichia coli | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| catalyses the rearrangement of -S-S- bonds in proteins | protein with nonconsecutive disulfie bonds require activity of DsbC for their full activity, the disulfide bonds are formed during the translocation across the cytoplasmic membrane | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Agp mutant | folding of Agp mutant, DsbC, mutant of Agp, an AppA homologue, containing the AppA nonconsecutive disulfide bond | Escherichia coli | ? | - |
? |  |
| additional information | substrate disulfide bonds, overview, the AppA homologue Agp, a periplasmic phosphatase, lacks nonconsecutive disulfide bonds and is no substrate for DsbC | Escherichia coli | ? | - |
? | |
| phytase | folding of phytase, i.e. AppA, substrate from Escherichia coli, contains 3 consecutive and 1 nonconsecutive disulfide bonds, DsbC, no activity of DsbC with an AppA mutant C155S/C430S lacking the nonconsecutive disulfide bond | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| disulfide bond isomerase | - |
Escherichia coli |
| DsbA | - |
Escherichia coli |
| DsbC | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
assay at with substrate AppA, DsbC | Escherichia coli |
| 4.5 | - |
assay at with substrate Agp mutant, DsbC | Escherichia coli |
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