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Literature summary for 5.3.4.1 extracted from

  • Rubotham, J.; Woods, K.; Garcia-Salcedo, J.A.; Pays, E.; Nolan, D.P.
    Characterization of two protein disulfide isomerases from the endocytic pathway of bloodstream forms of Trypanosoma brucei (2005), J. Biol. Chem., 280, 10410-10418.
    View publication on PubMed
Search for more literature for 5.3.4.1

Activating Compound

Activating Compound Comment Organism Structure
DTT required for activity Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination of PDI-1 and PDI-2, which show low overall identity, genomic organization and expression analysis in Trypanosoma brucei, expression of both enzyme forms as GST-tagged proteins in Escherichia coli Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information gene silencing of PDI-1 and PDI-2 by RNAi constructs does not influence the cell growth Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information both enzyme forms co-localized with markers of the endocytic pathway and concentrate at the posterior end of the cell between the nucleus and the kinetoplast Trypanosoma brucei
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, recombinant PDI-1, SDS-PAGE, x * 80000, recombinant PDI-2, SDS-PAGE Trypanosoma brucei
80000
-
 x * 45000, recombinant PDI-1, SDS-PAGE, x * 80000, recombinant PDI-2, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei the single domain PDI-1 and the class 1 PDI-2 are not essential for the organism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei P12865 PDI-2; two PDI enzyme forms, which contain one or two double-cysteine redox-active sites and correspond to a single domain PDI and a class 1 PDI, i.e. PDI-1 and PDI-2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both enzyme forms are N-glycosylated, PDI-1 contains 2 N-glycosylation sites, PDI-2 contains 12 sites Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
additional information developmentally regulated expression of both PDI enzyme forms, the enzymes are not essential for growth of trypanosomes Trypanosoma brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
degenerated RNase type III refolding of degenerated RNase type III, bovine pancreatic substrate, recombinant GST-tagged PDI, the coupled-assay method involves reduction of insulin in presence of DTT Trypanosoma brucei ?
-
 ?
additional information the single domain PDI-1 and the class 1 PDI-2 are not essential for the organism Trypanosoma brucei ?
-
 ?
additional information the single domain PDI-1 and the class 1 PDI-2 of the organism both posses isomerase activity, but only the single domain PDI has reducing activity Trypanosoma brucei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, recombinant PDI-1, SDS-PAGE, x * 80000, recombinant PDI-2, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
protein disulfide isomerase
-
 Trypanosoma brucei