Any feedback?
Literature summary for 5.3.4.1 extracted from
- Inactivation of protein disulfide isomerase by alkylators including alpha,beta-unsaturated aldehydes at low physiological pHs (2004), Biol. Chem., 385, 633-637.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxy-2-nonenal | 44% inhibition at 0.03 mM | Bos taurus |  |
| acrolein | 79% inhibition at 0.03 mM | Bos taurus | |
| iodoacetamide | pH-dependent inactivation | Bos taurus | |
| iodoacetate | - |
Bos taurus | |
| additional information | malonaldehyde bis(diethyl acetal) is a poor inhibitor, pH-dependency of inhibition by alkylating inhibitors and SH-reagents | Bos taurus | |
| N-ethylmaleimide | pH-independent inactivation within the range of pH 6.3-7.0 | Bos taurus | |
| tert-2-hexenal | 32% inhibition at 1 mM | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from liver | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin | reduction of insulin, turbidometry assay including glutathione reduction and alkylation of 4-acetamido-4'-maleimidyl-stilbene-2,2'-disulfonate, as well as treatment with DTT and iodoacetamide or iodoacetate for subsequent insulin reduction, isomerase activity, overview | Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDI | - |
Bos taurus |
| protein disulfide isomerase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pseudo-first-order and second-order kinetics | Bos taurus | |
| 0.124 | - |
N-ethylmaleimide | pH 6.3, 38°C | Bos taurus | |
| 0.179 | - |
iodoacetate | pH 6.3, 38°C | Bos taurus | |
| 0.311 | - |
iodoacetamide | pH 6.3, 38°C | Bos taurus | |
| 0.447 | - |
acrolein | pH 6.3, 38°C | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
