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Literature summary for 5.3.3.8 extracted from

  • Yang, S.Y.; He, X.Y.; Schulz, H.
    Glutamate 139 of the large alpha-subunit is the catalytic base in the dehydration of both D- and L-3-hydroxyacyl-coenzyme A but not in the isomerization of DELTA3,DELTA2-enoyl-coenzyme A catalyzed by the multienzyme complex of fatty acid oxidation from Escherichia coli (1995), Biochemistry, 34, 6441-6447.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
E134Q catalytic properties of the five different component enzyme activities of the alpha/Arg134Gln mutant complex show no significant changes as compared with those of the wild-type complex. In the alpha/Glu139Gln mutant complex DELTA3-cis-DELTA2-trans-enoyl-CoA isomerase shows 60% decreased turnover number and a significant increase in Km for 3-cis-tetradecenoyl-CoA Escherichia coli
E139Q The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 3-cis-tetradecenoyl-CoA wild-type enzyme complex Escherichia coli
0.089
-
 3-cis-tetradecenoyl-CoA mutant enzyme complex alpha/Glu139Gln Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 wild-type and mutant enzymes alpha/Glu139Gln and alphaArg134Gln with mutations in the large alpha-subunit
-
Escherichia coli
-
 multienzyme complex which exhibits activity of EC 4.2.1.17, EC 1.1.1.35, EC 2.3.1.16, EC 5.1.2.3 and EC 5.3.3.8
-

Purification (Commentary)

Purification (Comment) Organism
partial, multienzyme complex of fatty acid oxidation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cis-Tetradecenoyl-CoA
-
 Escherichia coli 2-trans-Tetradecenoyl-CoA
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
 3-cis-tetradecenoyl-CoA mutant enzyme complex alpha/Glu139Gln Escherichia coli
63
-
 3-cis-tetradecenoyl-CoA wild-type enzyme complex Escherichia coli