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Literature summary for 5.3.3.8 extracted from
- Purification and characterization of bovine liver 3-cis-2-trans-enoyl-CoA isomerase (1990), Biol. Chem. Hoppe-Seyler, 371, 603-610.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-trans-Dodecenoic acid | competitive inhibitor of 3-cis-dodecenoyl-CoA | Bos taurus |  |
| 3-Dodecynoic acid | competitive inhibitor of 3-cis-dodecenoyl-CoA | Bos taurus | |
| CoA | competitive inhibitor of 3-cis-dodecenoyl-CoA | Bos taurus | |
| diethyldicarbonate | - |
Bos taurus | |
| PCMB | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
3-trans-dodecenoyl-CoA | - |
Bos taurus | |
| 0.032 | - |
3-cis-dodecenoyl-CoA | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Bos taurus |
| 60000 | - |
gel filtration | Sus scrofa |
| 60000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| loss of activity by photooxidation of His residue at neutral pH in presence of rose bengal | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cis-dodecenoyl-CoA | - |
Sus scrofa | 2-trans-dodecenoyl-CoA | - |
? | |
| 3-cis-dodecenoyl-CoA | - |
Bos taurus | 2-trans-dodecenoyl-CoA | - |
? | |
| 3-trans-dodecenoyl-CoA | - |
Sus scrofa | 2-trans-dodecenoyl-CoA | - |
? | |
| 3-trans-dodecenoyl-CoA | - |
Bos taurus | 2-trans-dodecenoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Sus scrofa |
| dimer | 2 * 30000, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 128 | - |
3-cis-dodecenoyl-CoA | - |
Bos taurus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5: about 50% of maximal activity, pH 7-9: maximal activity | Bos taurus |
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