Literature summary for 5.3.3.5 extracted from

Moebius, F.F.; Soellner, K.E.M.; Fiechtner, B.; Huck, C.W.; Bonn, G.; Glossmann, H.
Histidine77, glutamic acid81, glutamic acid123, threonine126, asparagine194, and tryptophan197 of the human emopamil binding protein are required for in vivo sterol DELTA8-DELTA7 isomerization (1999), Biochemistry, 38, 1119-1127.

Protein Variants

Protein Variants	Comment	Organism
C121A	mutant enzyme with 95% of the in vivo activity of the wild-type enzyme	Homo sapiens
C128A	mutant enzyme with 81% of the in vivo activity of the wild-type enzyme	Homo sapiens
C68A	mutant enzyme with 94% of the in vivo activity of the wild-type enzyme	Homo sapiens
C73A	mutant enzyme with 83% of the in vivo activity of the wild-type enzyme	Homo sapiens
E123A	mutant enzyme with 10% of the in vivo activity of the wild-type enzyme	Homo sapiens
E123D	mutant enzyme does not bind the inhibitor ifenprodil	Homo sapiens
E81A	mutant enzyme with 5% of the in vivo activity of the wild-type enzyme	Homo sapiens
E81D	mutant enzyme still binds the inhibitor ifenprodil	Homo sapiens
F186A	mutant enzyme with 56% of the in vivo activity of the wild-type enzyme	Homo sapiens
F188A	mutant enzyme with 94% of the in vivo activity of the wild-type enzyme	Homo sapiens
F190A	mutant enzyme with 21% of the in vivo activity of the wild-type enzyme	Homo sapiens
F192A	mutant enzyme with 92% of the in vivo activity of the wild-type enzyme	Homo sapiens
F37A	mutant enzyme with 74% of the in vivo activity of the wild-type enzyme	Homo sapiens
F70A	mutant enzyme with 95% of the in vivo activity of the wild-type enzyme	Homo sapiens
F75A	mutant enzyme with 88% of the in vivo activity of the wild-type enzyme	Homo sapiens
F84A	mutant enzyme with 72% of the in vivo activity of the wild-type enzyme	Homo sapiens
G131A	mutant enzyme with 69% of the in vivo activity of the wild-type enzyme	Homo sapiens
G41A	mutant enzyme with 94% of the in vivo activity of the wild-type enzyme	Homo sapiens
G82A/F84V	mutant enzyme with 56% of the in vivo activity of the wild-type enzyme	Homo sapiens
H31A	mutant enzyme with 69% of the in vivo activity of the wild-type enzyme	Homo sapiens
H77A	mutant enzyme with 9% of the in vivo activity of the wild-type enzyme	Homo sapiens
H77N	mutant enzyme does not bind the inhibitor ifenprodil	Homo sapiens
I125A	mutant enzyme with 78% of the in vivo activity of the wild-type enzyme	Homo sapiens
I139A	mutant enzyme with 90% of the in vivo activity of the wild-type enzyme	Homo sapiens
I32A	mutant enzyme with 73% of the in vivo activity of the wild-type enzyme	Homo sapiens
I76A	mutant enzyme with 33% of the in vivo activity of the wild-type enzyme	Homo sapiens
I76L/L78A	mutant enzyme with 74% of the in vivo activity of the wild-type enzyme	Homo sapiens
L129A	mutant enzyme with 81% of the in vivo activity of the wild-type enzyme	Homo sapiens
L133A	mutant enzyme with 77% of the in vivo activity of the wild-type enzyme	Homo sapiens
L135A	mutant enzyme with 92% of the in vivo activity of the wild-type enzyme	Homo sapiens
L196A	mutant enzyme with 62% of the in vivo activity of the wild-type enzyme	Homo sapiens
L198A	mutant enzyme with 50% of the in vivo activity of the wild-type enzyme	Homo sapiens
L204A	mutant enzyme with 77% of the in vivo activity of the wild-type enzyme	Homo sapiens
L33A	mutant enzyme with 47% of the in vivo activity of the wild-type enzyme	Homo sapiens
L35A	mutant enzyme with 62% of the in vivo activity of the wild-type enzyme	Homo sapiens
L43A	mutant enzyme with 103% of the in vivo activity of the wild-type enzyme	Homo sapiens
L49A	mutant enzyme with 108% of the in vivo activity of the wild-type enzyme	Homo sapiens
L50A	mutant enzyme with 96% of the in vivo activity of the wild-type enzyme	Homo sapiens
L65A	mutant enzyme with 82% of the in vivo activity of the wild-type enzyme	Homo sapiens
L67A	mutant enzyme with 63% of the in vivo activity of the wild-type enzyme	Homo sapiens
M122A	mutant enzyme with 28% of the in vivo activity of the wild-type enzyme	Homo sapiens
M193A	mutant enzyme with 53% of the in vivo activity of the wild-type enzyme	Homo sapiens
N194Q	mutant enzyme still binds the inhibitor ifenprodil	Homo sapiens
S134A	mutant enzyme with 101% of the in vivo activity of the wild-type enzyme	Homo sapiens
S38A	mutant enzyme with 74% of the in vivo activity of the wild-type enzyme	Homo sapiens
T124A	mutant enzyme with 91% of the in vivo activity of the wild-type enzyme	Homo sapiens
T126A	mutant enzyme with 4% of the in vivo activity of the wild-type enzyme	Homo sapiens
T126S	mutant enzyme still binds the inhibitor ifenprodil	Homo sapiens
V120A	mutant enzyme with 62% of the in vivo activity of the wild-type enzyme	Homo sapiens
V138A	mutant enzyme with 94% of the in vivo activity of the wild-type enzyme	Homo sapiens
V191A	mutant enzyme with 91% of the in vivo activity of the wild-type enzyme	Homo sapiens
V199A	mutant enzyme with 76% of the in vivo activity of the wild-type enzyme	Homo sapiens
V203A	mutant enzyme with 71% of the in vivo activity of the wild-type enzyme	Homo sapiens
V39A	mutant enzyme with 80% of the in vivo activity of the wild-type enzyme	Homo sapiens
V44A	mutant enzyme with 76% of the in vivo activity of the wild-type enzyme	Homo sapiens
V45A	mutant enzyme with 79% of the in vivo activity of the wild-type enzyme	Homo sapiens
V72A	mutant enzyme with 93% of the in vivo activity of the wild-type enzyme	Homo sapiens
V79A	mutant enzyme with 75% of the in vivo activity of the wild-type enzyme	Homo sapiens
V85A	mutant enzyme with 68% of the in vivo activity of the wild-type enzyme	Homo sapiens
W130A	mutant enzyme with 70% of the in vivo activity of the wild-type enzyme	Homo sapiens
W136A	mutant enzyme with 104% of the in vivo activity of the wild-type enzyme	Homo sapiens
W187A	mutant enzyme with 56% of the in vivo activity of the wild-type enzyme	Homo sapiens
W197A	mutant enzyme with 5% of the in vivo activity of the wild-type enzyme	Homo sapiens
W197F	mutant enzyme still binds the inhibitor ifenprodil	Homo sapiens
W48A	mutant enzyme with 87% of the in vivo activity of the wild-type enzyme	Homo sapiens
W69A	mutant enzyme with 17% of the in vivo activity of the wild-type enzyme	Homo sapiens
W83A	mutant enzyme with 97% of the in vivo activity of the wild-type enzyme	Homo sapiens
Y185A	mutant enzyme with 64% of the in vivo activity of the wild-type enzyme	Homo sapiens
Y189A	mutant enzyme with 32% of the in vivo activity of the wild-type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
ifenprodil	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q15125	-	-

Synonyms

Synonyms	Comment	Organism
EBP	-	Homo sapiens
Emopamil binding protein	-	Homo sapiens