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Literature summary for 5.3.3.2 extracted from

  • Sharma, N.K.; Pan, J.J.; Poulter, C.D.
    Type II isopentenyl diphosphate isomerase: probing the mechanism with alkyne/allene diphosphate substrate analogues (2010), Biochemistry, 49, 6228-6233.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermus thermophilus
expressed in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2,3-butadien-1-yl diphosphate competitive inhibitor Escherichia coli
2,3-butadien-1-yl diphosphate competitive inhibitor Thermus thermophilus
3-butyn-1-yl diphosphate competitive inhibitor Escherichia coli
3-butyn-1-yl diphosphate competitive inhibitor Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Thermus thermophilus
Mg2+ required for activity Escherichia coli
Zn2+ IDI-1 is a zinc-metalloprotein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
 Thermus thermophilus dimethylallyl diphosphate
-
 r
isopentenyl diphosphate
-
 Escherichia coli dimethylallyl diphosphate
-
 r
additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-1 Escherichia coli ?
-
 ?
additional information 3-butyn-1-yl diphosphate and 2,3-butadien-1-yl diphosphate are no substrates for IDI-2 Thermus thermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
IDI-1
-
 Escherichia coli
Idi-2
-
 Thermus thermophilus
type I isopentenyl diphosphate isomerase
-
 Escherichia coli
type II isopentenyl diphosphate isomerase
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
FMN IDI-2 requires reduced flavin Thermus thermophilus
NADPH
-
 Thermus thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.031
-
 2,3-butadien-1-yl diphosphate in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C Escherichia coli
0.036
-
 2,3-butadien-1-yl diphosphate in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C Thermus thermophilus
0.048
-
 3-butyn-1-yl diphosphate in 200 mM HEPES (pH 7.0) containing 2 mM MgCl2, 0.04 mM FMN, 2 mM NADPH, at 37°C Thermus thermophilus
0.049
-
 3-butyn-1-yl diphosphate in 50 mM HEPES (pH 7.2) containing 10 mM MgCl2, 200 mM KCl, 1 mg/ml bovine serum albumin, 0.5 mM dithiothreitol, at 37°C Escherichia coli