Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.6 M calcium acetate and 50 mM HEPES pH 7.5 | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-epoxy-3-methylbutyl diphosphate | irreversible inhibition | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | IDI-2 requires a divalent cation such as Mg2+ for activity | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography and Resource Phe column chromatograpyh | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Methanocaldococcus jannaschii | dimethylallyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
Idi-2 | - |
Methanocaldococcus jannaschii |
type 2 isopentenyl diphosphate isomerase | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | required, IDI-2 reconstituted with cofactor analogues such as 5-deaza-FMN show no activity | Methanocaldococcus jannaschii |