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Literature summary for 5.3.3.2 extracted from

  • Steinbacher, S.; Kaiser, J.; Gerhardt, S.; Eisenreich, W.; Huber, R.; Bacher, A.; Rohdich, F.
    Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis (2003), J. Mol. Biol., 329, 973-982.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, ligand-free form of the FMN-bound enzyme form at 2.8 A resolution. The octamer forms a D4 symmetrical open, cage-like structure. The monomers of 45000 Da display a classical TIM barrel fold Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
8 * 45000 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P50740 type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase
-

Subunits

Subunits Comment Organism
octamer 8 * 45000 Bacillus subtilis

Synonyms

Synonyms Comment Organism
IDI
-
Bacillus subtilis
isopentenyl diphosphate:dimethylallyl diphosphate isomerase
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMN bound only with very moderate affinity and is therefore completely lost during purification. However, the enzyme can be reconstituted in the crystals by soaking with FMN Bacillus subtilis