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Literature summary for 5.3.3.2 extracted from

  • Yamashita, S.; Hemmi, H.; Ikeda, Y.; Nakayama, T.; Nishino, T.
    Type 2 isopentenyl diphosphate isomerase from a thermoacidophilic archaeon Sulfolobus shibatae (2004), Eur. J. Biochem., 271, 1087-1093.
    View publication on PubMed
Search for more literature for 5.3.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus shibatae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 isopentenyl diphosphate pH 6.0, 60°C Saccharolobus shibatae
0.0874
-
 NADH dehydrogenase reaction without isopentenyl diphosphate Saccharolobus shibatae
0.0904
-
 NADH isomerase reaction Saccharolobus shibatae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae P61615 type 2 isopentenyl diphosphate isomerase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate
-
 Saccharolobus shibatae dimethylallyl diphosphate
-
 ?
additional information the enzyme also has NADH dehydrogenase activity although it catalyzes the isomerase reaction without consuming any detectable amount of NADH. O2 acts as electron acceptor in the NADH dehydrogenase reaction Saccharolobus shibatae ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Saccharolobus shibatae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 isopentenyl diphosphate pH 6.0, 60°C Saccharolobus shibatae

Cofactor

Cofactor Comment Organism Structure
FMN strictly dependent on Saccharolobus shibatae
NAD(P)H required Saccharolobus shibatae