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Literature summary for 5.3.3.2 extracted from

  • Ramos-Valdivia, A.C.; van der Heijden, R.; Verpoorte, R.; Camara, B.
    Purification and characterization of two isoforms of isopentenyl-diphosphate isomerase from elicitor-treated Cinchona robusta cells (1997), Eur. J. Biochem., 249, 161-170.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
2-(dimethylamino)ethyl phosphate
-
 Cinchona robusta
geranyl diphosphate
-
 Cinchona robusta
Mg2+ isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory Cinchona robusta
Mn2+ isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory Cinchona robusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 isopentenyl diphosphate isomerase II Cinchona robusta
0.0051
-
 isopentenyl diphosphate isomerase I Cinchona robusta
0.017
-
 dimethylallyl diphosphate isomerase I Cinchona robusta

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ or Mg2+ required Cinchona robusta
Mg2+ activation by a mixture of Mg2+ and Mn2+ gives the highest activity Cinchona robusta
Mg2+ isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory Cinchona robusta
Mg2+ isomerase II: maximal activation at 2-8 mM Mg2+, the maximal activation is 35% lower than the activation obtained with Mn2+ Cinchona robusta
Mn2+ Mn2+ or Mg2+ required Cinchona robusta
Mn2+ activation by a mixture of Mg2+ and Mn2+ gives the highest activity Cinchona robusta
Mn2+ isomerase I is activated by concentrations below 1 mM of Mn2+ or Mg2+, increasing concentrations are inhibitory Cinchona robusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, isomerase I, SDS-PAGE Cinchona robusta
34000
-
 x * 34000, isomerase II, SDS-PAGE Cinchona robusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cinchona robusta induced after addition of a homogenate of the phytopathogenic fungus Phytophthora cinnamomi. Enzyme may be involved in the biosynthesis of anthraquinone phytoalexins that accumulate rapidly after elicitation of Cinchona cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cinchona robusta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 isoforms: I and II Cinchona robusta

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Cinchona robusta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.046
-
-
 Cinchona robusta

Storage Stability

Storage Stability Organism
-80°C, 0.05 M Tris/HCl, pH 7.5, containing 0.01 mM leupeptin, 2 mM dithiothreitol, 5% glycerol, 55% of the activity is recovered after 3 months Cinchona robusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate r Cinchona robusta dimethylallyl diphosphate
-
 ?
additional information induced after addition of a homogenate of the phytopathogenic fungus Phytophthora cinnamomi. Enzyme may be involved in the biosynthesis of anthraquinone phytoalexins that accumulate rapidly after elicitation of Cinchona cells Cinchona robusta ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, isomerase I, SDS-PAGE Cinchona robusta
? x * 34000, isomerase II, SDS-PAGE Cinchona robusta

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0-7.8: maximal activity, pH 8.5: 20% of maximal activity, isomerase I Cinchona robusta