Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridioides difficile |
Crystallization (Comment) | Organism |
---|---|
molecular dockng using L-xylose and D-ribose as the substrate. Residue M95 seems to be a determinant residue for the specificity on free sugars | Clostridioides difficile |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not affected by metal ions and not inhibited by EDTA | Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | 20 | L-talose | pH 7.5, 40°C | Clostridioides difficile | |
30 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile | |
270 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
460 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17200 | - |
2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
17217 | - |
2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
34000 | - |
gel filtration | Clostridioides difficile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridioides difficile | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | ? | - |
? | |
additional information | Clostridioides difficile ATCC BAA-1382D-5 | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | - |
- |
- |
Clostridioides difficile ATCC BAA-1382D-5 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
substrate D-sorbose, pH 7.5, 40°C | Clostridioides difficile |
3 | - |
substrate D-psicose, pH 7.5, 40°C | Clostridioides difficile |
7 | - |
substrate L-lyxose, pH 7.5, 40°C | Clostridioides difficile |
9 | - |
substrate L-fructose, pH 7.5, 40°C | Clostridioides difficile |
14 | - |
substrate D-ribulose, pH 7.5, 40°C | Clostridioides difficile |
15 | - |
substrate D-allose, pH 7.5, 40°C | Clostridioides difficile |
28 | - |
substrate L-talose, pH 7.5, 40°C | Clostridioides difficile |
45 | - |
substrate L-talose, pH 7.5, 40°C | Clostridioides difficile |
53 | - |
substrate D-ribose, pH 7.5, 40°C | Clostridioides difficile |
57 | - |
substrate L-xylulose, pH 7.5, 40°C | Clostridioides difficile |
120 | - |
substrate D-gulose, pH 7.5, 40°C | Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Clostridioides difficile | D-psicose | - |
r | |
D-allose | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-psicose | - |
r | |
D-gulose | - |
Clostridioides difficile | D-sorbose | - |
r | |
D-psicose | - |
Clostridioides difficile | D-allose | - |
r | |
D-ribose | - |
Clostridioides difficile | D-ribulose | - |
r | |
D-ribose | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-ribulose | - |
r | |
D-ribose 5-phosphate | - |
Clostridioides difficile | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-ribulose 5-phosphate | - |
? | |
D-ribulose | - |
Clostridioides difficile | D-ribose | - |
r | |
D-sorbose | - |
Clostridioides difficile | D-gulose | - |
r | |
L-fructose | - |
Clostridioides difficile | L-mannose | - |
r | |
L-lyxose | - |
Clostridioides difficile | L-xylulose | - |
r | |
L-lyxose | - |
Clostridioides difficile ATCC BAA-1382D-5 | L-xylulose | - |
r | |
L-tagatose | - |
Clostridioides difficile | L-talose | - |
r | |
L-talose | - |
Clostridioides difficile | L-tagatose | - |
r | |
L-xylulose | - |
Clostridioides difficile | L-lyxose | - |
r | |
additional information | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | ? | - |
? | |
additional information | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
Synonyms | Comment | Organism |
---|---|---|
RpiB | - |
Clostridioides difficile |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Clostridioides difficile |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life 53 min | Clostridioides difficile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
L-talose | pH 7.5, 40°C | Clostridioides difficile | |
160 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
200 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile | |
15100 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Clostridioides difficile |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridioides difficile | isoelectric focusing | - |
6.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-talose | pH 7.5, 40°C | Clostridioides difficile | |
0.4 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile | |
0.6 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
500 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile |