Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.6 extracted from

  • Wang, C.; Fan, X.; Cao, X.; Liu, X.; Li, L.; Su, X.
    Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans (2012), Acta Crystallogr. Sect. F, 68, 565-568.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 0.1 M NaCl, 2.0 M ammonium sulfate Streptococcus mutans
sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M Tris-HCl pH 8.5 and 25% (w/v) PEG 3350, 0.2 M MgCl2, 0.1 M Tris-HCl pH 8.5 Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23400
-
 x * 23400, isoform RpiB, calculated from amino acid sequence Streptococcus mutans
24570
-
 x * 24570, isoform RpiA, calculated from amino acid sequence Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribose 5-phosphate Streptococcus mutans
-
 D-ribulose 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q8DRS5
-
-
Streptococcus mutans Q8DTT9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating column chromatography and Superdex S75 gel filtration Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
 Streptococcus mutans D-ribulose 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 23400, isoform RpiB, calculated from amino acid sequence Streptococcus mutans
? x * 24570, isoform RpiA, calculated from amino acid sequence Streptococcus mutans

Synonyms

Synonyms Comment Organism
RpiA isoform Streptococcus mutans
RpiB isoform Streptococcus mutans
SMU1234 isoform Streptococcus mutans
SMU2142 isoform Streptococcus mutans