Literature summary for 5.3.1.6 extracted from

Kim, T.G.; Kwon, T.H.; Min, K.; Dong, M.S.; Park, Y.I.; Ban, C.
Crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase A from Vibrio vulnificus YJ016 (2009), Mol. Cells, 27, 99-103.

Cloned(Commentary)

Cloned (Comment)	Organism
gene rpiA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Vibrio vulnificus

Crystallization (Commentary)

Crystallization (Comment)	Organism
open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement	Vibrio vulnificus

Crystallization (Comment)

Organism

open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement

Vibrio vulnificus

Inhibitors

Inhibitors	Comment	Organism	Structure
arabinose 5-phosphate	RpiA binds arabinose 5-phosphate located at the opening of the active site, the sugar ring of the inhibitor interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues, the phosphate group of arabinose 5-phosphate interacts with two water molecules, W51 and W82, binding mode of inhibitor, overview	Vibrio vulnificus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	recombinant detagged RpiA, dynamic light scattering and gel filtration	Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-ribose 5-phosphate	Vibrio vulnificus	-	D-ribulose 5-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus	Q7MHL9	gene rpiA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration	Vibrio vulnificus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-ribose 5-phosphate	-	Vibrio vulnificus	D-ribulose 5-phosphate	-	r
D-ribose 5-phosphate	binding mode of substrate, overview	Vibrio vulnificus	D-ribulose 5-phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 20000-25000, RpiA	Vibrio vulnificus

Synonyms

Synonyms	Comment	Organism
ribose 5-phosphate isomerase A	-	Vibrio vulnificus
RpiA	-	Vibrio vulnificus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio vulnificus	isoelectric focusing	-	4.77