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Literature summary for 5.3.1.6 extracted from

  • Roos, A.K.; Mariano, S.; Kowalinski, E.; Salmon, L.; Mowbray, S.L.
    D-ribose-5-phosphate isomerase B from Escherichia coli is also a functional D-allose-6-phosphate isomerase, while the Mycobacterium tuberculosis enzyme is not (2008), J. Mol. Biol., 382, 667-679.
    View publication on PubMed
Search for more literature for 5.3.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 5-deoxy-5-phospho-D-ribonohydroxamate, hanging drop vapour diffusion method, with 15% PEG 8000, 0.1 M MES buffer, pH 6, 5% PEG 1000, and 0.2 M Li2SO4, or in complex with D-ribose 5-phosphate, sitting drop vapour diffusion method, with 20% PEG 3K, 0.1 M Tris, pH 7, and 0.2 M Ca acetate Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
5-deoxy-5-phospho-D-ribonohydroxamate
-
 Mycobacterium tuberculosis
D-Allose 6-phosphate
-
 Mycobacterium tuberculosis
D-allulose 6-phosphate
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 D-ribose 5-phosphate in 50 mM Tris-HCl, pH 7.5, at 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis P9WKD7
-
-
Mycobacterium tuberculosis H37Rv P9WKD7
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
 Escherichia coli D-ribulose 5-phosphate
-
 ?
D-ribose 5-phosphate
-
 Mycobacterium tuberculosis D-ribulose 5-phosphate
-
 ?
D-ribose 5-phosphate
-
 Mycobacterium tuberculosis H37Rv D-ribulose 5-phosphate
-
 ?
additional information no activity with D-allose 6-phosphate Mycobacterium tuberculosis ?
-
 ?
additional information no activity with D-allose 6-phosphate Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
D-ribose-5-phosphate isomerase A
-
 Escherichia coli
D-ribose-5-phosphate isomerase B
-
 Mycobacterium tuberculosis
RpiA
-
 Escherichia coli
RpiB
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47
-
 D-ribose 5-phosphate in 50 mM Tris-HCl, pH 7.5, at 37°C Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2
-
 in 50 mM Tris-HCl, pH 7.5, at 37°C Mycobacterium tuberculosis D-Allose 6-phosphate
6.3
-
 in 50 mM Tris-HCl, pH 7.5, at 37°C Mycobacterium tuberculosis D-allulose 6-phosphate