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Literature summary for 5.3.1.6 extracted from

  • Middaugh, C.R.; MacElroy, R.D.
    The effect of temperature on ribose-5-phosphate isomerase from mesophile Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus (1976), J. Biochem., 79, 1331-1344.
    View publication on PubMed
Search for more literature for 5.3.1.6

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Thiobacillus thioparus
2-mercaptoethanol stimulates [Bacillus] caldolyticus
EDTA stimulates Thiobacillus thioparus
EDTA stimulates [Bacillus] caldolyticus

General Stability

General Stability Organism
(NH4)2SO4, NaCl, KCl and LiCl increase thermal stability. LiBr, CaCl2, methanol, ethanol, and 1-propanol decrease thermal stability. Alcohols decrease the stability in the following order: methanol, ethanol, propanol Thiobacillus thioparus
(NH4)2SO4, NaCl, KCl and LiCl increase thermal stability. LiBr, CaCl2, methanol, ethanol, and 1-propanol decrease thermal stability. Alcohols decrease the stability in the following order: methanol, ethanol, propanol [Bacillus] caldolyticus
ethylene glycol has little effect on the mesophilic enzyme Thiobacillus thioparus
ethylene glycol increases stability [Bacillus] caldolyticus

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate temperature-dependent inhibition [Bacillus] caldolyticus
6-phosphogluconate temperature-dependent inhibition Thiobacillus thioparus
Ag+ 1 mM [Bacillus] caldolyticus
Ag+ 1 mM Thiobacillus thioparus
AMP temperature-dependent inhibition [Bacillus] caldolyticus
AMP temperature-dependent inhibition Thiobacillus thioparus
Co2+ 1 mM [Bacillus] caldolyticus
Co2+ 1 mM Thiobacillus thioparus
Mg2+ 0.01 M [Bacillus] caldolyticus
Mg2+ 0.01 M Thiobacillus thioparus
Phenylmercuriacetate reversible by 0.02-10 mM 2-mercaptoethanol, and by 10 mM Cys [Bacillus] caldolyticus
Phenylmercuriacetate reversible by 0.02-10 mM 2-mercaptoethanol, and by 10 mM Cys Thiobacillus thioparus
Sodium mersalyl reversible by 2-mercaptoethanol, 0.02-10 mM, and by Cys, 10 mM [Bacillus] caldolyticus
Sodium mersalyl reversible by 2-mercaptoethanol, 0.02-10 mM, and by Cys, 10 mM Thiobacillus thioparus
Zn2+ 0.01 M [Bacillus] caldolyticus
Zn2+ 0.01 M Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Thiobacillus thioparus
40000
-
 gel filtration [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thiobacillus thioparus
-
 [Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.7
-
-
 [Bacillus] caldolyticus
544
-
-
 Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
 Thiobacillus thioparus D-ribulose 5-phosphate
-
 ?
D-ribose 5-phosphate
-
 [Bacillus] caldolyticus D-ribulose 5-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 5-10, stable for at least 30 min Thiobacillus thioparus
57
-
 1 h, 50% loss of activity Thiobacillus thioparus
65
-
 pH 5-10, stable for at least 30 min [Bacillus] caldolyticus
91
-
 1 h, 50% loss of activity [Bacillus] caldolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9
-
 Thiobacillus thioparus
6.5 9
-
 [Bacillus] caldolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 65°C, stable for at least 30 min [Bacillus] caldolyticus
5 10 45°C, stable for at least 30 min Thiobacillus thioparus