Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2 (DE3)pLysS cells | Bacteroides thetaiotaomicron |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM sodium chloride, 100 mM bicine pH 9.0, 20% (v/v) PEG MME 550 | Bacteroides thetaiotaomicron |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | Bacteroides thetaiotaomicron | - |
D-Fructose | - |
r | |
D-Glucose | Bacteroides thetaiotaomicron DSM 2079 | - |
D-Fructose | - |
r | |
D-Xylose | Bacteroides thetaiotaomicron | - |
D-Xylulose | - |
r | |
D-Xylose | Bacteroides thetaiotaomicron DSM 2079 | - |
D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q8A9M2 | - |
- |
Bacteroides thetaiotaomicron DSM 2079 | Q8A9M2 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Bacteroides thetaiotaomicron | D-Fructose | - |
r | |
D-Glucose | - |
Bacteroides thetaiotaomicron DSM 2079 | D-Fructose | - |
r | |
D-Xylose | - |
Bacteroides thetaiotaomicron | D-Xylulose | - |
r | |
D-Xylose | - |
Bacteroides thetaiotaomicron DSM 2079 | D-Xylulose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Bacteroides thetaiotaomicron |