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Literature summary for 5.3.1.5 extracted from

  • Ravikumar, S.; Vikramathithan, J.; Srikumar, K.
    Purification and characterization of a novel thermostable xylose isomerase from Opuntia vulgaris Mill (2011), Appl. Biochem. Biotechnol., 164, 593-603.
    View publication on PubMed
Search for more literature for 5.3.1.5

Application

Application Comment Organism
food industry thermophilic xylose isomerase from Opuntia vulgaris can serve as a good alternate source of enzyme for use in the production of high fructose corn syrup Opuntia ficus-indica
food industry thermophilic xylose isomerase from Opuntia vulgaris can serve as a good alternate source of enzyme for use in the production of high fructose corn syrup Opuntia vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
46.4
-
 D-xylose enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia ficus-indica
46.4
-
 D-xylose enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia vulgaris
259.6
-
 D-glucose enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia ficus-indica
259.6
-
 D-glucose enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ purified T90 isoform, 100% activity at 10 mM Mn2+ plus 1 mM Co2+ Opuntia ficus-indica
Co2+ purified T90 isoform, 100% activity at 10 mM Mn2+ plus 1 mM Co2+ Opuntia vulgaris
Mn2+ purified T90 isoform, 100% activity at 10 mM Mn2+ plus 1 mM Co2+ Opuntia ficus-indica
Mn2+ purified T90 isoform, 100% activity at 10 mM Mn2+ plus 1 mM Co2+ Opuntia vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 1 * 66000, SDS-PAGE Opuntia ficus-indica
66000
-
 1 * 66000, SDS-PAGE Opuntia vulgaris
68000
-
 purified T90 isoform, gel filtration Opuntia ficus-indica
68000
-
 purified T90 isoform, gel filtration Opuntia vulgaris
71000
-
 purified T70 isoform, gel filtration Opuntia ficus-indica
71000
-
 purified T70 isoform, gel filtration Opuntia vulgaris

Organism

Organism UniProt Comment Textmining
Opuntia ficus-indica
-
-
-
Opuntia vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration Opuntia ficus-indica
ammonium sulfate precipitation, Dowex-1 anion exchange column chromatography, and Sephadex G-100 gel filtration Opuntia vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.78
-
 enzyme isoform T90 from homogenate with xylose as a substrate, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia ficus-indica
6.78
-
 enzyme isoform T90 from homogenate with xylose as a substrate, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia vulgaris
266.4
-
 enzyme isoform T90 after 39.2fold purification with xylose as a substrate, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia ficus-indica
266.4
-
 enzyme isoform T90 after 39.2fold purification with xylose as a substrate, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C Opuntia vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
 Opuntia ficus-indica D-Fructose
-
 ?
D-Glucose
-
 Opuntia vulgaris D-Fructose
-
 ?
D-Xylose
-
 Opuntia ficus-indica D-Xylulose
-
 ?
D-Xylose
-
 Opuntia vulgaris D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Opuntia ficus-indica
monomer 1 * 66000, SDS-PAGE Opuntia vulgaris

Synonyms

Synonyms Comment Organism
D-xylose ketol-isomerase
-
 Opuntia ficus-indica
D-xylose ketol-isomerase
-
 Opuntia vulgaris
xylose isomerase xylose isomerase exhibits two temperature stable isoforms having optimal activity at temperatures 70°C (T70) and 90°C (T90), respectively Opuntia ficus-indica
xylose isomerase xylose isomerase exhibits two temperature stable isoforms having optimal activity at temperatures 70°C (T70) and 90°C (T90), respectively Opuntia vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 purified T70 isoform Opuntia ficus-indica
70
-
 purified T70 isoform Opuntia vulgaris
90
-
 purified T90 isoform Opuntia ficus-indica
90
-
 purified T90 isoform Opuntia vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.5
-
 the purified T90 isoform at pH 7.5 exhibits a preference to yield the forward isomerization reaction Opuntia ficus-indica
7.5
-
 the purified T90 isoform at pH 7.5 exhibits a preference to yield the forward isomerization reaction Opuntia vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 the melting temperature of the native enzyme isoform T90 is at 90°C Opuntia ficus-indica
90
-
 the melting temperature of the native enzyme isoform T90 is at 90°C Opuntia vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 purified T90 isoform Opuntia ficus-indica
6 8 purified T90 isoform Opuntia vulgaris
7.5
-
 purified T70 isoform Opuntia ficus-indica
7.5
-
 purified T70 isoform Opuntia vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 the enzyme retains 60% of its activity even at pH 4.5 Opuntia ficus-indica
4 8.5 the enzyme retains 60% of its activity even at pH 4.5 Opuntia vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 the enzyme exhibits a broad range (5.5-8.5) of pH stability but denatures readily at pH values below 4.0 Opuntia ficus-indica
5.5 8.5 the enzyme exhibits a broad range (5.5-8.5) of pH stability but denatures readily at pH values below 4.0 Opuntia vulgaris