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Literature summary for 5.3.1.5 extracted from

  • Xu, W.; Yan, M.; Xu, L.; Ding, L.; Ouyang, P.
    Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces (2009), Enzyme Microb. Technol., 44, 77-83.
No PubMed abstract available
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes in Escherichia coli strain BL21 (DE3) Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
N91D site-directed mutagenesis Thermus thermophilus
N91D/D375G site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
N91D/D375G/V385A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
N91D/K355A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
N91D/V144A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.7
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
11.8
-
 D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus
15.2
-
 D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
21.1
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
27.1
-
 D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Thermus thermophilus
-
 D-Xylulose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P26997
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
 Thermus thermophilus D-Xylulose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 temperature profiles of recombinant mutant enzymes, overview Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 90 thermostability of recombinant mutant enzymes, overview Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50.7
-
 D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
61.65
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
68.39
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
116
-
 D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus
160.5
-
 D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH profiles of recombinant mutant enzymes, overview Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.92
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
3.35
-
 D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
5.87
-
 D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
5.92
-
 D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus
9.8
-
 D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus