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Literature summary for 5.3.1.5 extracted from

  • Lopes, M.S.; Gomez, J.G.; Silva, L.F.
    Cloning and overexpression of the xylose isomerase gene from Burkholderia sacchari and production of polyhydroxybutyrate from xylose (2009), Can. J. Microbiol., 55, 1012-1015.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xylA, expression of the polyhydroxybutyrate producing enzyme in an enzyme-deficient knockout strain of Burkholderia sacchari restoring the ability of the cells to produce polyhydroxybutyrate and increasing cell growth, overview Paraburkholderia sacchari

Protein Variants

Protein Variants Comment Organism
additional information overexpression of the polyhydroxybutyrate producing enzyme in an enzyme-deficient knockout strain of Burkholderia sacchari restoring the ability of the cells to produce polyhydroxybutyrate, overview. Expression in a wild-type strain does not lead to increased polyhydroxybutyrate repoduction and cell growth, overview Paraburkholderia sacchari

Organism

Organism UniProt Comment Textmining
Paraburkholderia sacchari B6VCW7 gene xylA
-
Paraburkholderia sacchari IPT101 B6VCW7 gene xylA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.46
-
wild-type strain Paraburkholderia sacchari
2.44
-
recombinant overexpressing mutant strain Paraburkholderia sacchari