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Literature summary for 5.3.1.5 extracted from

  • Joo, G.J.; Shin, J.H.; Heo, G.Y.; Kwak, Y.Y.; Choi, J.H.; Rhee, I.K.
    Purification and characterization of a thermostable xylose (glucose) isomerase from Streptomyces chibaensis J-59 (2001), Agric. Chem. Biotechnol., 44, 113-118.
No PubMed abstract available
Search for more literature for 5.3.1.5

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Streptomyces corchorusii
Ca2+ no inhibitory effect Streptomyces corchorusii
Cu2+
-
 Streptomyces corchorusii
Hg2+
-
 Streptomyces corchorusii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
83
-
 D-glucose
-
 Streptomyces corchorusii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required for activity Streptomyces corchorusii
Mg2+ required for activity, most effective cation Streptomyces corchorusii
Mn2+ required for activity Streptomyces corchorusii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 SDS-PAGE, monomer Streptomyces corchorusii
180000
-
 gel filtration Streptomyces corchorusii

Organism

Organism UniProt Comment Textmining
Streptomyces corchorusii
-
-
-
Streptomyces corchorusii J-59
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Streptomyces corchorusii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.9
-
-
 Streptomyces corchorusii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
 Streptomyces corchorusii D-Fructose
-
 ?
D-Glucose
-
 Streptomyces corchorusii J-59 D-Fructose
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 44000, SDS-PAGE, gel filtration Streptomyces corchorusii

Synonyms

Synonyms Comment Organism
xylose (glucose) isomerase
-
 Streptomyces corchorusii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Streptomyces corchorusii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 in the presence of CO2+, cell-free xylose isomerase retains 100% activity without loss of activity for 7 days Streptomyces corchorusii
85
-
 in the presence of CO2+, cell-free xylose isomerase retains 50% residual activity for 13.5 h Streptomyces corchorusii
90
-
 in the presence of CO2+, cell-free xylose isomerase retains 50% residual activity for 126 min Streptomyces corchorusii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.72
-
 D-glucose
-
 Streptomyces corchorusii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces corchorusii