Application | Comment | Organism |
---|---|---|
synthesis | commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses | Bacillus licheniformis |
synthesis | commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses | Streptomyces olivochromogenes |
synthesis | commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses | Actinoplanes missouriensis |
synthesis | commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses | Streptomyces wedmorensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
expression in Escherichia coli | Bacillus licheniformis |
expression in Escherichia coli | Actinoplanes missouriensis |
expression in Escherichia coli | Streptomyces griseofuscus |
expression in Escherichia coli | Thermoanaerobacter thermohydrosulfuricus |
expression in Escherichia coli | Actinoplanes sp. |
expression in Escherichia coli or Bacillus subtilis | Thermoanaerobacterium thermosulfurigenes |
Protein Variants | Comment | Organism |
---|---|---|
E253K | substitution of Arg for Lys at position 253 at the dimer-dimer interface increases the half-life of the enzyme by 30%. The largest stability gain is achieved in a triple mutant G70S/A73S/G74T | Actinoplanes missouriensis |
H101F | - |
Thermoanaerobacterium thermosulfurigenes |
H101F | substitution of His101 by Phe abolishes the enzyme activity, whereas substitution of other His residues has no effect | Actinoplanes missouriensis |
H101X | selective substitution of His101 or His271 shows that they are essential components of the active site | Escherichia coli |
H271X | selective substitution of His101 or His271 shows that they are essential components of the active site | Escherichia coli |
W139A | replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid | Thermoanaerobacterium thermosulfurigenes |
W139F | replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid | Thermoanaerobacterium thermosulfurigenes |
W139F | the W139F substitution reduces the Km and increases the turnover number of the mutant towards glucose, while the reverse effect towards xylose is observed | Thermoanaerobacterium thermosulfurigenes |
W139F/V186S | double mutants, W139F/V186T and W139F/V186S have 5fold and 2fold higher catalytic efficiency, respectively, than does the wild-type | Thermoanaerobacterium thermosulfurigenes |
W139F/V186T | double mutants, W139F/V186T and W139F/V186S have 5fold and 2fold higher catalytic efficiency, respectively, than does the wild-type | Thermoanaerobacterium thermosulfurigenes |
W139M | replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid | Thermoanaerobacterium thermosulfurigenes |
Z180L | one of the metal-binding sites, M-1, is removed by substitution of Glu-180 by Lys. Glu-180 is essential for isomerization but not for ring opening | Streptomyces olivochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces olivocinereus | - |
- |
- |
Actinoplanes missouriensis | - |
- |
- |
Actinoplanes sp. | - |
- |
- |
Aerobacter levanicum | - |
- |
- |
Aeromonas hydrophila | - |
- |
- |
Arthrobacter sp. | - |
- |
- |
Bacillus licheniformis | - |
- |
- |
Brevibacterium helvolum | - |
- |
- |
Brevibacterium pentosoaminoacidicum | - |
- |
- |
Citrobacter freundii | - |
- |
- |
Citrobacter intermedius | - |
- |
- |
Corynebacterium sp. | - |
- |
- |
Desemzia incerta | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Escherichia coli | - |
- |
- |
Gordonia rubripertincta | - |
- |
- |
Klebsiella aerogenes | - |
- |
- |
Lactiplantibacillus pentosus | - |
- |
- |
Lactiplantibacillus plantarum | - |
- |
- |
Lactobacillus gayonii | - |
- |
- |
Lactobacillus lycopersici | - |
- |
- |
Lactobacillus mannitopoeus | - |
- |
- |
Lentilactobacillus buchneri | - |
- |
- |
Leuconostoc mesenteroides | - |
- |
- |
Limosilactobacillus fermentum | - |
- |
- |
Microbacterium arborescens | - |
- |
- |
Microbispora rosea | - |
- |
- |
Micromonospora coerulea | - |
- |
- |
Mycobacterium sp. | - |
- |
- |
Nocardia asteroides | - |
- |
- |
Nocardiopsis dassonvillei | - |
- |
- |
Paracolobacterium aerogenoides | - |
- |
- |
Priestia megaterium | - |
- |
- |
Pseudonocardia sp. | - |
- |
- |
Sarcina sp. | - |
- |
- |
Sphingomonas paucimobilis | - |
- |
- |
Streptococcus venuceus | - |
- |
- |
Streptomyces achromogenes | - |
- |
- |
Streptomyces bikiniensis | - |
- |
- |
Streptomyces bobili | - |
- |
- |
Streptomyces californicus | - |
- |
- |
Streptomyces echinatus | - |
- |
- |
Streptomyces flaveus | - |
- |
- |
Streptomyces flavovirens | - |
- |
- |
Streptomyces fradiae | - |
- |
- |
Streptomyces glaucescens | - |
- |
- |
Streptomyces griseofuscus | - |
- |
- |
Streptomyces griseolus | - |
- |
- |
Streptomyces griseus | - |
- |
- |
Streptomyces matensis | - |
- |
- |
Streptomyces nivens | - |
- |
- |
Streptomyces olivaceus | - |
- |
- |
Streptomyces olivochromogenes | - |
- |
- |
Streptomyces phaeochromogenes | - |
- |
- |
Streptomyces platensis | - |
- |
- |
Streptomyces roseochromogenus | - |
- |
- |
Streptomyces rubiginosus | - |
- |
- |
Streptomyces venezuelae | - |
- |
- |
Streptomyces virginiae | - |
- |
- |
Streptomyces wedmorensis | - |
- |
- |
Streptosporangium album | - |
- |
- |
Streptosporangium vulgare | - |
- |
- |
Thermoanaerobacter thermohydrosulfuricus | - |
- |
- |
Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Thermopolyspora sp. | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Weizmannia coagulans | - |
- |
- |
Xanthomonas sp. | - |
- |
- |
Zymomonas mobilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Lactiplantibacillus plantarum | D-Xylulose | - |
? | |
D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
? | |
D-Xylose | - |
Escherichia coli | D-Xylulose | - |
? | |
D-Xylose | - |
Enterobacter cloacae | D-Xylulose | - |
? | |
D-Xylose | - |
Priestia megaterium | D-Xylulose | - |
? | |
D-Xylose | - |
Klebsiella aerogenes | D-Xylulose | - |
? | |
D-Xylose | - |
Corynebacterium sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Leuconostoc mesenteroides | D-Xylulose | - |
? | |
D-Xylose | - |
Weizmannia coagulans | D-Xylulose | - |
? | |
D-Xylose | - |
Bacillus licheniformis | D-Xylulose | - |
? | |
D-Xylose | - |
Arthrobacter sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Mycobacterium sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Limosilactobacillus fermentum | D-Xylulose | - |
? | |
D-Xylose | - |
Aeromonas hydrophila | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces griseus | D-Xylulose | - |
? | |
D-Xylose | - |
Lactobacillus gayonii | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces olivochromogenes | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces rubiginosus | D-Xylulose | - |
? | |
D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? | |
D-Xylose | - |
Thermoanaerobacterium thermosulfurigenes | D-Xylulose | - |
? | |
D-Xylose | - |
Actinomyces olivocinereus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces phaeochromogenes | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces olivaceus | D-Xylulose | - |
? | |
D-Xylose | - |
Aerobacter levanicum | D-Xylulose | - |
? | |
D-Xylose | - |
Desemzia incerta | D-Xylulose | - |
? | |
D-Xylose | - |
Brevibacterium pentosoaminoacidicum | D-Xylulose | - |
? | |
D-Xylose | - |
Brevibacterium helvolum | D-Xylulose | - |
? | |
D-Xylose | - |
Citrobacter freundii | D-Xylulose | - |
? | |
D-Xylose | - |
Citrobacter intermedius | D-Xylulose | - |
? | |
D-Xylose | - |
Microbacterium arborescens | D-Xylulose | - |
? | |
D-Xylose | - |
Sphingomonas paucimobilis | D-Xylulose | - |
? | |
D-Xylose | - |
Lentilactobacillus buchneri | D-Xylulose | - |
? | |
D-Xylose | - |
Lactobacillus mannitopoeus | D-Xylulose | - |
? | |
D-Xylose | - |
Lactobacillus lycopersici | D-Xylulose | - |
? | |
D-Xylose | - |
Lactiplantibacillus pentosus | D-Xylulose | - |
? | |
D-Xylose | - |
Microbispora rosea | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces flaveus | D-Xylulose | - |
? | |
D-Xylose | - |
Micromonospora coerulea | D-Xylulose | - |
? | |
D-Xylose | - |
Nocardia asteroides | D-Xylulose | - |
? | |
D-Xylose | - |
Gordonia rubripertincta | D-Xylulose | - |
? | |
D-Xylose | - |
Nocardiopsis dassonvillei | D-Xylulose | - |
? | |
D-Xylose | - |
Paracolobacterium aerogenoides | D-Xylulose | - |
? | |
D-Xylose | - |
Pseudonocardia sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Sarcina sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces bobili | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces flavovirens | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces echinatus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces achromogenes | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces fradiae | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces roseochromogenus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces californicus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptococcus venuceus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces virginiae | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces venezuelae | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces wedmorensis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces griseolus | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces glaucescens | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces bikiniensis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces nivens | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces matensis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces platensis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptosporangium album | D-Xylulose | - |
? | |
D-Xylose | - |
Streptosporangium vulgare | D-Xylulose | - |
? | |
D-Xylose | - |
Thermopolyspora sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Xanthomonas sp. | D-Xylulose | - |
? | |
D-Xylose | - |
Zymomonas mobilis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces griseofuscus | D-Xylulose | - |
? | |
D-Xylose | - |
Thermoanaerobacter thermohydrosulfuricus | D-Xylulose | - |
? | |
D-Xylose | - |
Actinoplanes sp. | D-Xylulose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Maxazyme | Gist Brocades and Anheuser-Busch Inc. | Actinoplanes missouriensis |
Optisweet | Miles Kali-Chemie | Streptomyces rubiginosus |
Spezyme | Finnsugar | Streptomyces rubiginosus |
Sweetzyme | Novo-Nordisk | Weizmannia coagulans |