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Literature summary for 5.3.1.5 extracted from

  • Bhosale, S.H.; Rao, M.B.; Deshpande, V.V.
    Molecular industrial aspects of glucose isomerase (1996), Microbiol. Rev., 60, 280-300.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses Bacillus licheniformis
synthesis commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses Streptomyces olivochromogenes
synthesis commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses Actinoplanes missouriensis
synthesis commercial importance in the production of high-fructose corn syrup, potential application in the production of ethanol from hemicelluloses Streptomyces wedmorensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus
expression in Escherichia coli Bacillus licheniformis
expression in Escherichia coli Actinoplanes missouriensis
expression in Escherichia coli Streptomyces griseofuscus
expression in Escherichia coli Thermoanaerobacter thermohydrosulfuricus
expression in Escherichia coli Actinoplanes sp.
expression in Escherichia coli or Bacillus subtilis Thermoanaerobacterium thermosulfurigenes

Protein Variants

Protein Variants Comment Organism
E253K substitution of Arg for Lys at position 253 at the dimer-dimer interface increases the half-life of the enzyme by 30%. The largest stability gain is achieved in a triple mutant G70S/A73S/G74T Actinoplanes missouriensis
H101F
-
Thermoanaerobacterium thermosulfurigenes
H101F substitution of His101 by Phe abolishes the enzyme activity, whereas substitution of other His residues has no effect Actinoplanes missouriensis
H101X selective substitution of His101 or His271 shows that they are essential components of the active site Escherichia coli
H271X selective substitution of His101 or His271 shows that they are essential components of the active site Escherichia coli
W139A replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid Thermoanaerobacterium thermosulfurigenes
W139F replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid Thermoanaerobacterium thermosulfurigenes
W139F the W139F substitution reduces the Km and increases the turnover number of the mutant towards glucose, while the reverse effect towards xylose is observed Thermoanaerobacterium thermosulfurigenes
W139F/V186S double mutants, W139F/V186T and W139F/V186S have 5fold and 2fold higher catalytic efficiency, respectively, than does the wild-type Thermoanaerobacterium thermosulfurigenes
W139F/V186T double mutants, W139F/V186T and W139F/V186S have 5fold and 2fold higher catalytic efficiency, respectively, than does the wild-type Thermoanaerobacterium thermosulfurigenes
W139M replacement of W139 with F, M, or A results in increased catalytic efficience proportional to the decrease in hydrophobicity of the side chain of the substituted amino acid Thermoanaerobacterium thermosulfurigenes
Z180L one of the metal-binding sites, M-1, is removed by substitution of Glu-180 by Lys. Glu-180 is essential for isomerization but not for ring opening Streptomyces olivochromogenes

Organism

Organism UniProt Comment Textmining
Actinomyces olivocinereus
-
-
-
Actinoplanes missouriensis
-
-
-
Actinoplanes sp.
-
-
-
Aerobacter levanicum
-
-
-
Aeromonas hydrophila
-
-
-
Arthrobacter sp.
-
-
-
Bacillus licheniformis
-
-
-
Brevibacterium helvolum
-
-
-
Brevibacterium pentosoaminoacidicum
-
-
-
Citrobacter freundii
-
-
-
Citrobacter intermedius
-
-
-
Corynebacterium sp.
-
-
-
Desemzia incerta
-
-
-
Enterobacter cloacae
-
-
-
Escherichia coli
-
-
-
Gordonia rubripertincta
-
-
-
Klebsiella aerogenes
-
-
-
Lactiplantibacillus pentosus
-
-
-
Lactiplantibacillus plantarum
-
-
-
Lactobacillus gayonii
-
-
-
Lactobacillus lycopersici
-
-
-
Lactobacillus mannitopoeus
-
-
-
Lentilactobacillus buchneri
-
-
-
Leuconostoc mesenteroides
-
-
-
Limosilactobacillus fermentum
-
-
-
Microbacterium arborescens
-
-
-
Microbispora rosea
-
-
-
Micromonospora coerulea
-
-
-
Mycobacterium sp.
-
-
-
Nocardia asteroides
-
-
-
Nocardiopsis dassonvillei
-
-
-
Paracolobacterium aerogenoides
-
-
-
Priestia megaterium
-
-
-
Pseudonocardia sp.
-
-
-
Sarcina sp.
-
-
-
Sphingomonas paucimobilis
-
-
-
Streptococcus venuceus
-
-
-
Streptomyces achromogenes
-
-
-
Streptomyces bikiniensis
-
-
-
Streptomyces bobili
-
-
-
Streptomyces californicus
-
-
-
Streptomyces echinatus
-
-
-
Streptomyces flaveus
-
-
-
Streptomyces flavovirens
-
-
-
Streptomyces fradiae
-
-
-
Streptomyces glaucescens
-
-
-
Streptomyces griseofuscus
-
-
-
Streptomyces griseolus
-
-
-
Streptomyces griseus
-
-
-
Streptomyces matensis
-
-
-
Streptomyces nivens
-
-
-
Streptomyces olivaceus
-
-
-
Streptomyces olivochromogenes
-
-
-
Streptomyces phaeochromogenes
-
-
-
Streptomyces platensis
-
-
-
Streptomyces roseochromogenus
-
-
-
Streptomyces rubiginosus
-
-
-
Streptomyces venezuelae
-
-
-
Streptomyces virginiae
-
-
-
Streptomyces wedmorensis
-
-
-
Streptosporangium album
-
-
-
Streptosporangium vulgare
-
-
-
Thermoanaerobacter thermohydrosulfuricus
-
-
-
Thermoanaerobacterium thermosulfurigenes
-
-
-
Thermopolyspora sp.
-
-
-
Thermus thermophilus
-
-
-
Weizmannia coagulans
-
-
-
Xanthomonas sp.
-
-
-
Zymomonas mobilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
Lactiplantibacillus plantarum D-Xylulose
-
?
D-Xylose
-
Thermus thermophilus D-Xylulose
-
?
D-Xylose
-
Escherichia coli D-Xylulose
-
?
D-Xylose
-
Enterobacter cloacae D-Xylulose
-
?
D-Xylose
-
Priestia megaterium D-Xylulose
-
?
D-Xylose
-
Klebsiella aerogenes D-Xylulose
-
?
D-Xylose
-
Corynebacterium sp. D-Xylulose
-
?
D-Xylose
-
Leuconostoc mesenteroides D-Xylulose
-
?
D-Xylose
-
Weizmannia coagulans D-Xylulose
-
?
D-Xylose
-
Bacillus licheniformis D-Xylulose
-
?
D-Xylose
-
Arthrobacter sp. D-Xylulose
-
?
D-Xylose
-
Mycobacterium sp. D-Xylulose
-
?
D-Xylose
-
Limosilactobacillus fermentum D-Xylulose
-
?
D-Xylose
-
Aeromonas hydrophila D-Xylulose
-
?
D-Xylose
-
Streptomyces griseus D-Xylulose
-
?
D-Xylose
-
Lactobacillus gayonii D-Xylulose
-
?
D-Xylose
-
Streptomyces olivochromogenes D-Xylulose
-
?
D-Xylose
-
Streptomyces rubiginosus D-Xylulose
-
?
D-Xylose
-
Actinoplanes missouriensis D-Xylulose
-
?
D-Xylose
-
Thermoanaerobacterium thermosulfurigenes D-Xylulose
-
?
D-Xylose
-
Actinomyces olivocinereus D-Xylulose
-
?
D-Xylose
-
Streptomyces phaeochromogenes D-Xylulose
-
?
D-Xylose
-
Streptomyces olivaceus D-Xylulose
-
?
D-Xylose
-
Aerobacter levanicum D-Xylulose
-
?
D-Xylose
-
Desemzia incerta D-Xylulose
-
?
D-Xylose
-
Brevibacterium pentosoaminoacidicum D-Xylulose
-
?
D-Xylose
-
Brevibacterium helvolum D-Xylulose
-
?
D-Xylose
-
Citrobacter freundii D-Xylulose
-
?
D-Xylose
-
Citrobacter intermedius D-Xylulose
-
?
D-Xylose
-
Microbacterium arborescens D-Xylulose
-
?
D-Xylose
-
Sphingomonas paucimobilis D-Xylulose
-
?
D-Xylose
-
Lentilactobacillus buchneri D-Xylulose
-
?
D-Xylose
-
Lactobacillus mannitopoeus D-Xylulose
-
?
D-Xylose
-
Lactobacillus lycopersici D-Xylulose
-
?
D-Xylose
-
Lactiplantibacillus pentosus D-Xylulose
-
?
D-Xylose
-
Microbispora rosea D-Xylulose
-
?
D-Xylose
-
Streptomyces flaveus D-Xylulose
-
?
D-Xylose
-
Micromonospora coerulea D-Xylulose
-
?
D-Xylose
-
Nocardia asteroides D-Xylulose
-
?
D-Xylose
-
Gordonia rubripertincta D-Xylulose
-
?
D-Xylose
-
Nocardiopsis dassonvillei D-Xylulose
-
?
D-Xylose
-
Paracolobacterium aerogenoides D-Xylulose
-
?
D-Xylose
-
Pseudonocardia sp. D-Xylulose
-
?
D-Xylose
-
Sarcina sp. D-Xylulose
-
?
D-Xylose
-
Streptomyces bobili D-Xylulose
-
?
D-Xylose
-
Streptomyces flavovirens D-Xylulose
-
?
D-Xylose
-
Streptomyces echinatus D-Xylulose
-
?
D-Xylose
-
Streptomyces achromogenes D-Xylulose
-
?
D-Xylose
-
Streptomyces fradiae D-Xylulose
-
?
D-Xylose
-
Streptomyces roseochromogenus D-Xylulose
-
?
D-Xylose
-
Streptomyces californicus D-Xylulose
-
?
D-Xylose
-
Streptococcus venuceus D-Xylulose
-
?
D-Xylose
-
Streptomyces virginiae D-Xylulose
-
?
D-Xylose
-
Streptomyces venezuelae D-Xylulose
-
?
D-Xylose
-
Streptomyces wedmorensis D-Xylulose
-
?
D-Xylose
-
Streptomyces griseolus D-Xylulose
-
?
D-Xylose
-
Streptomyces glaucescens D-Xylulose
-
?
D-Xylose
-
Streptomyces bikiniensis D-Xylulose
-
?
D-Xylose
-
Streptomyces nivens D-Xylulose
-
?
D-Xylose
-
Streptomyces matensis D-Xylulose
-
?
D-Xylose
-
Streptomyces platensis D-Xylulose
-
?
D-Xylose
-
Streptosporangium album D-Xylulose
-
?
D-Xylose
-
Streptosporangium vulgare D-Xylulose
-
?
D-Xylose
-
Thermopolyspora sp. D-Xylulose
-
?
D-Xylose
-
Xanthomonas sp. D-Xylulose
-
?
D-Xylose
-
Zymomonas mobilis D-Xylulose
-
?
D-Xylose
-
Streptomyces griseofuscus D-Xylulose
-
?
D-Xylose
-
Thermoanaerobacter thermohydrosulfuricus D-Xylulose
-
?
D-Xylose
-
Actinoplanes sp. D-Xylulose
-
?

Synonyms

Synonyms Comment Organism
Maxazyme Gist Brocades and Anheuser-Busch Inc. Actinoplanes missouriensis
Optisweet Miles Kali-Chemie Streptomyces rubiginosus
Spezyme Finnsugar Streptomyces rubiginosus
Sweetzyme Novo-Nordisk Weizmannia coagulans