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Literature summary for 5.3.1.5 extracted from

  • Lehmbacher, A.; Bisswanger, H.
    Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB8 (1990), J. Gen. Microbiol., 136, 679-686.
No PubMed abstract available

General Stability

General Stability Organism
immobilized enzyme remains stable for at least 45 d at 4°C or at 25°C, upon incubation at 70°C the half-life is about 4 d Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, SDS-PAGE Thermus aquaticus
196000
-
gel filtration Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus aquaticus important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway ?
-
?
additional information Thermus aquaticus HB8 important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus HB8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus aquaticus

Storage Stability

Storage Stability Organism
4°C, stable for more than 1 month Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Thermus aquaticus D-Fructose
-
?
D-Glucose
-
Thermus aquaticus HB8 D-Fructose
-
?
D-Xylose
-
Thermus aquaticus D-Xylulose
-
?
D-Xylose
-
Thermus aquaticus HB8 D-Xylulose
-
?
additional information important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway Thermus aquaticus ?
-
?
additional information important enzyme in degradation of xylans and metabolizing D-xylose via the pentose phosphate pathway Thermus aquaticus HB8 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
stable for more than 1 month Thermus aquaticus
70
-
half-life in absence of divalent cations: 4 d. In presence of Mn2+ or Co2+ stable for at least 1 month Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.5
-
Thermus aquaticus