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Literature summary for 5.3.1.5 extracted from

  • Suekane, M.; Tamura, M.; Tomimura, Ch.
    Physico-chemical and enzymatic properties of purified glucose isomerases from Streptomyces olivochromogenes and Bacillus stearothermophilus (1978), Agric. Biol. Chem., 42, 909-917.
No PubMed abstract available
Search for more literature for 5.3.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Streptomyces olivochromogenes

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Geobacillus stearothermophilus
Cu2+
-
 Streptomyces olivochromogenes
Mn2+
-
 Streptomyces olivochromogenes
Ni2+
-
 Geobacillus stearothermophilus
Ni2+
-
 Streptomyces olivochromogenes
Sn2+
-
 Geobacillus stearothermophilus
Zn2+
-
 Geobacillus stearothermophilus
Zn2+
-
 Streptomyces olivochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18
-
 xylulose
-
 Streptomyces olivochromogenes
33
-
 D-xylose
-
 Streptomyces olivochromogenes
100
-
 D-xylulose
-
 Geobacillus stearothermophilus
200
-
 D-fructose
-
 Geobacillus stearothermophilus
200
-
 D-fructose
-
 Streptomyces olivochromogenes
220
-
 D-fructose
-
 Streptomyces olivochromogenes
250
-
 D-glucose
-
 Streptomyces olivochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE Streptomyces olivochromogenes
115000 117000 low speed sedimentation without reaching equilibrium, equilibrium sedimentation Streptomyces olivochromogenes
127000
-
 equilibrium sedimentation Geobacillus stearothermophilus
130000
-
 1 * 130000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Streptomyces olivochromogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus
-
 Streptomyces olivochromogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Streptomyces olivochromogenes
2.7
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose
-
 Geobacillus stearothermophilus ?
-
 ?
D-Fructose
-
 Streptomyces olivochromogenes ?
-
 ?
D-Glucose
-
 Geobacillus stearothermophilus D-Fructose
-
 ?
D-Glucose
-
 Streptomyces olivochromogenes D-Fructose
-
 ?
D-Xylose
-
 Geobacillus stearothermophilus D-Xylulose
-
 ?
D-Xylose r Streptomyces olivochromogenes D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Streptomyces olivochromogenes
monomer 1 * 130000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Geobacillus stearothermophilus
80
-
-
 Streptomyces olivochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable Streptomyces olivochromogenes
65
-
 30 min, about 50% loss of activity Streptomyces olivochromogenes
70
-
-
 Geobacillus stearothermophilus
70
-
 30 min, about 70% loss of activity Streptomyces olivochromogenes
75
-
 60 min, stable up to Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Geobacillus stearothermophilus
8 10
-
 Streptomyces olivochromogenes

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 35% of maximal activity, pH 10: about 30% of maximal activity Streptomyces olivochromogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 room temperature, 30 min, stable above Streptomyces olivochromogenes
5
-
 room temperature, 30 min, stable above Geobacillus stearothermophilus