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Literature summary for 5.3.1.27 extracted from
- Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007), Appl. Microbiol. Biotechnol., 76, 439-445.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fusion gene construct of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. The gene product of 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase exhibits both activities at room temperature and catalyzes the sequential reactions more efficiently than a simple mixture of the individual enzymes. The gene product of 6-phospho-3-hexuloisomerase /3-hexulose-6-phosphate synthase fails to display any enzyme activity. Escherichia coli strains harboring the 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase gene consume formaldehyde more efficiently and exhibited better growth in a formaldehyde containing medium than the host strain | Mycobacterium gastri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
| 42000 | - |
x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme | Mycobacterium gastri |
| 94000 | - |
gel filtration, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium gastri | Q9LBW5 | - |
- |
| Mycobacterium gastri MB19 | Q9LBW5 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 154 | - |
3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerasefusion enzyme, 30°C | Mycobacterium gastri |
| 563 | - |
6-phospho-3-hexuloisomerase, 30°C | Mycobacterium gastri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabino-3-hexulose 6-phosphate | - |
Mycobacterium gastri | D-fructose 6-phosphate | - |
? |  |
| D-arabino-3-hexulose 6-phosphate | - |
Mycobacterium gastri MB19 | D-fructose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme | Mycobacterium gastri |
| tetramer | 4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
both recombinant 6-phospho-3-hexuloisomerase and 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase fusion enzyme | Mycobacterium gastri |
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