Literature summary for 5.3.1.1 extracted from

Nguyen, T.N.; Abendroth, J.; Leibly, D.J.; Le, K.P.; Guo, W.; Kelley, A.; Stewart, L.; Myler, P.J.; Van Voorhis, W.C.
Structure of triosephosphate isomerase from Cryptosporidium parvum (2011), Acta Crystallogr. Sect. F, 67, 1095-1099.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells	Cryptosporidium parvum

Crystallization (Commentary)

Crystallization (Comment)	Organism
using 100 mM Bis-Tris propane pH 7.5, 200 mM sodium sulfate, 20% (w/v) PEG 3350	Cryptosporidium parvum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	Cryptosporidium parvum	-	dihydroxyacetone phosphate	-	r
dihydroxyacetone phosphate	Cryptosporidium parvum	-	D-glyceraldehyde 3-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Cryptosporidium parvum	Q5CSE7	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography Superdex 75 and gel filtration	Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Cryptosporidium parvum	dihydroxyacetone phosphate	-	r
dihydroxyacetone phosphate	-	Cryptosporidium parvum	D-glyceraldehyde 3-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
TIM	-	Cryptosporidium parvum
Triosephosphate isomerase	-	Cryptosporidium parvum

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Release 2023.1 (January 2023)