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Literature summary for 5.3.1.1 extracted from

  • Connor, S.E.; Capodagli, G.C.; Deaton, M.K.; Pegan, S.D.
    Structural and functional characterization of Mycobacterium tuberculosis triosephosphate isomerase (2011), Acta Crystallogr. Sect. D, 67, 1017-1022.
    View publication on PubMed
Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 16% (w/v) PEG 3350 and 250 mM ammonium citrate Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 dihydroxyacetone phosphate in 100 mM Tris pH 7.8, at 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WG43
-
-
Mycobacterium tuberculosis H37Rv P9WG43
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-Bind resin column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
 Mycobacterium tuberculosis D-glyceraldehyde 3-phosphate
-
 ?
dihydroxyacetone phosphate
-
 Mycobacterium tuberculosis H37Rv D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
TpI
-
 Mycobacterium tuberculosis
Triosephosphate isomerase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
730
-
 dihydroxyacetone phosphate in 100 mM Tris pH 7.8, at 25°C Mycobacterium tuberculosis