Literature summary for 5.3.1.1 extracted from

Kumar, K.; Bhargava, P.; Roy, U.
In Vitro Refolding of Triosephosphate Isomerase from L. donovani (2011), Appl. Biochem. Biotechnol., 164, 1207-1214.

Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
in expression vector pET-23(a) with C-terminal His6 tag fused in frame, expressed in Escherichia coli BL21(DE3) cells	Leishmania donovani

General Stability

General Stability	Organism
bovine serum albunin assists in the refolding and regain of TIM enzyme activity by providing framework for structure formation	Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27600	-	x * 27600, SDS-PAGE	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	Q0ZAG6	-	-
Leishmania donovani MHOM/IN/80/Dd8	Q0ZAG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Leishmania donovani

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Leishmania donovani	dihydroxyacetone phosphate	-	?
D-glyceraldehyde 3-phosphate	-	Leishmania donovani MHOM/IN/80/Dd8	dihydroxyacetone phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 27600, SDS-PAGE	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
TIM	-	Leishmania donovani
Triosephosphate isomerase	-	Leishmania donovani

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Release 2023.1 (January 2023)