Crystallization (Comment) | Organism |
---|---|
purified enzyme mutant E65Q in complex with reaction-intermediate analogue phosphoglycolohydroxamate, hanging drop method, TIM-PGH crystals are grown at room temperature, 0.004 ml of protein solution with 11 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl, 10 mM PGH, 1 mM DTT, 1 mM EDTA, and 1 mM NaN3, are mixed with 0.004 ml of well solution containing 0.1M acetate, pH 5.0, 24% PEG6000, 1 mM DTT, 1 mM EDTA, and 1 mM NaN3, X-ray diffraction structure determination and analysis at 0.82 A resolution | Leishmania mexicana |
Protein Variants | Comment | Organism |
---|---|---|
E65Q | the variant of Leishmania mexicana TIM has a much enhanced stability but its catalytic properties are the same as wild-type leishmanial TIM | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phosphoglycolohydroxamate | the reaction-intermediate analogue binds to the active site with two hydrogen-bonding interactions between PGH and the Glu167 side-chain oxygen atoms | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Leishmania mexicana | the reaction is essential in vivo | Glycerone phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | P48499 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-Glyceraldehyde 3-phosphate = glycerone phosphate | reaction mechanism via enediolate intermediate through proton abstraction by the catalytic base Glu167, overview | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | the reaction is essential in vivo | Leishmania mexicana | Glycerone phosphate | - |
r | |
D-Glyceraldehyde 3-phosphate | triosephosphate isomerase is an enolizing enzyme, which catalyses the interconversion of dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate | Leishmania mexicana | Glycerone phosphate | - |
r | |
additional information | the proton transfer reaction from PGH to the Glu167 side chain, when PGH becomes sequestered in the active site, active site modeling, overview | Leishmania mexicana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | structure comparisons, overview | Leishmania mexicana |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Leishmania mexicana |
Triosephosphate isomerase | - |
Leishmania mexicana |
General Information | Comment | Organism |
---|---|---|
metabolism | the dimeric enzyme functions in the glycolytic pathway. In the glycolysis, the catalyzed interconversion reaction is important in the thermodynamically uphill direction of the synthesis of D-glyceraldehyde 3-phosphate | Leishmania mexicana |