Literature summary for 5.3.1.1 extracted from

Hernandez-Alcantara, G.; Rodriguez-Romero, A.; Reyes-Vivas, H.; Peon, J.; Cabrera, N.; Ortiz, C.; Enriquez-Flores, S.; De la Mora-De la Mora, I.; Lopez-Velazquez, G.
Unraveling the mechanisms of tryptophan fluorescence quenching in the triosephosphate isomerase from Giardia lamblia (2008), Biochim. Biophys. Acta, 1784, 1493-1500.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
determination of intrinsic fluorescence of wild-type and mutants lacking Trp residues, interpretation based on crystal structure. Fluorescence of all Trp residues is quenched by aromatic-aromatic interactions due to the proximity and orientation of the indole groups of Trp196 and Tro162. Quenching is also due to energy transfer to the charged resiudes that surround Trp75, Trp173, and Trp196	Giardia intestinalis

Protein Variants

Protein Variants	Comment	Organism
F12W	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W162F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W162F/W173F/W196F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W173F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W196F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W75F	mutant constructed for fluorescence quenching studies, catalytic properties similar to wild-type	Giardia intestinalis
W75F/W162F/W173F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W75F/W162F/W196F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis
W75F/W173F/W196F	mutant constructed for fluorescence studies, catalytic properties similar to wild-type	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.28	-	D-glyceraldehyde 3-phosphate	mutant W162F	Giardia intestinalis
0.43	-	D-glyceraldehyde 3-phosphate	mutant W196F	Giardia intestinalis
0.6	-	D-glyceraldehyde 3-phosphate	mutant F12W	Giardia intestinalis
0.73	-	D-glyceraldehyde 3-phosphate	mutant W75F/W162F/W196F	Giardia intestinalis
0.74	-	D-glyceraldehyde 3-phosphate	mutant W75F	Giardia intestinalis
0.78	-	D-glyceraldehyde 3-phosphate	wildtype	Giardia intestinalis
0.94	-	D-glyceraldehyde 3-phosphate	mutant W173F	Giardia intestinalis
1	-	D-glyceraldehyde 3-phosphate	mutant W162F/W173F/W196F	Giardia intestinalis
1.12	-	D-glyceraldehyde 3-phosphate	mutant W75F/W162F/W173F	Giardia intestinalis
1.9	-	D-glyceraldehyde 3-phosphate	mutant W75F/W173F/W196F	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	P36186	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Giardia intestinalis	dihydroxyacetone phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3166	-	D-glyceraldehyde 3-phosphate	mutant W162F/W173F/W196F	Giardia intestinalis
3333	-	D-glyceraldehyde 3-phosphate	mutant W162F	Giardia intestinalis
3666	-	D-glyceraldehyde 3-phosphate	mutant W75F/W162F/W173F	Giardia intestinalis
4166	-	D-glyceraldehyde 3-phosphate	mutant W196F	Giardia intestinalis
4333	-	D-glyceraldehyde 3-phosphate	mutant W75F/W173F/W196F	Giardia intestinalis
5500	-	D-glyceraldehyde 3-phosphate	mutant F12W	Giardia intestinalis
6166	-	D-glyceraldehyde 3-phosphate	mutant W173F	Giardia intestinalis
7166	-	D-glyceraldehyde 3-phosphate	mutant W75F/W162F/W196F	Giardia intestinalis
7666	-	D-glyceraldehyde 3-phosphate	wildtype	Giardia intestinalis
8000	-	D-glyceraldehyde 3-phosphate	mutant W75F	Giardia intestinalis

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Release 2023.1 (January 2023)