Protein Variants | Comment | Organism |
---|---|---|
C14S | wild-type-like enzyme that is resistant to the action of sulfhydryl reagents methylmethane thiosulfonate and 5,5-dithiobis(2-nitrobenzoate) | Trypanosoma brucei |
C14S/A69C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
C14S/A73C | greatly reduced kcat value. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
C14S/S71C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
C14S/S79C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A73C, 25°C, pH 7.4 | Trypanosoma brucei | |
0.35 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S71C, 25°C, pH 7.4 | Trypanosoma brucei | |
0.38 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.4 | Trypanosoma brucei | |
0.48 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S79C, 25°C, pH 7.4 | Trypanosoma brucei | |
0.5 | - |
D-glyceraldehyde 3-phosphate | mutant C14S, 25°C, pH 7.4 | Trypanosoma brucei | |
0.75 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A69C, 25°C, pH 7.4 | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | dihydroxyacetone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
483 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A73C, 25°C, pH 7.4 | Trypanosoma brucei | |
2600 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A69C, 25°C, pH 7.4 | Trypanosoma brucei | |
2967 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S79C, 25°C, pH 7.4 | Trypanosoma brucei | |
3200 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S71C, 25°C, pH 7.4 | Trypanosoma brucei | |
4183 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.4 | Trypanosoma brucei | |
5133 | - |
D-glyceraldehyde 3-phosphate | mutant C14S, 25°C, pH 7.4 | Trypanosoma brucei |