Protein Variants | Comment | Organism |
---|---|---|
C14A | the KM-value for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 90% of the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is comparable to the value for the wild-type enzyme | Trypanosoma brucei |
C14F | the Ki-value for 3-phosphoglycolate is nearly 3times higher than the Ki-value for the wild-type enzyme. The ratio of elimination to isomerization reactions is higher than in the wild-type enzyme. The KM-value for D-glyceraldehyde 3-phosphate is 10.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 2.7fold higher than the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 2280fold lower than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 3000fold lower than the value for the wild-type enzyme | Trypanosoma brucei |
C14P | the KM-value for D-glyceraldehyde 3-phosphate is 94% of the value for the wild-type enzyme. The KM-value for glycerone phosphate is 58% of the value for the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 85% of the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 81% of the value for the wild-type enzyme | Trypanosoma brucei |
C14S | the KM-value for D-glyceraldehyde 3-phosphate is 1.4fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is identical to the value of the wild-type value. The turnover number for D-glyceraldehyde 3-phosphate is 1.15fold higher than the value for the wild-type enzyme. The turnover-number for glycerone phosphate is nearly identical to the value of the wild-type enzyme | Trypanosoma brucei |
C14T | the KM-value for D-glyceraldehyde 3-phosphate is 1.2fold higher than the value for the wild-type enzyme. The KM-value for glycerone phosphate is 79% of the value of the wild-type enzyme. The turnover number for D-glyceraldehyde 3-phosphate is 92% of the value for the wild-type enzyme. The turnover-number for glycerone phosphate is 92% of the value of the wild-type enzyme | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phosphoglycolate | - |
Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14T | Trypanosoma brucei | |
0.33 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14P | Trypanosoma brucei | |
0.35 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, wild-type enzyme | Trypanosoma brucei | |
0.42 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14V | Trypanosoma brucei | |
0.43 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14A | Trypanosoma brucei | |
0.5 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14S | Trypanosoma brucei | |
3.6 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14F | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
mutant enzyme C14T, gel filtration | Trypanosoma brucei |
26879 | - |
1 * 26879, equilibrium sedimentation | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes C14A, C14P, C14S, C14T, C14V and C14G. Attempts to purify mutant enzymes C14M and C14L are unsuccessful, the enzymes exhibit a strong tendency to undergo aggregation | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | Glycerone phosphate | - |
r | |
glycerone phosphate | - |
Trypanosoma brucei | methylglyoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26879, equilibrium sedimentation | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14F | Trypanosoma brucei | |
3667 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14P | Trypanosoma brucei | |
3670 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14P | Trypanosoma brucei | |
3830 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14T | Trypanosoma brucei | |
3833 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14T | Trypanosoma brucei | |
4000 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14V | Trypanosoma brucei | |
4330 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, wild-type enzyme | Trypanosoma brucei | |
4333 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, wild-type enzyme | Trypanosoma brucei | |
5000 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14S | Trypanosoma brucei | |
5167 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14A | Trypanosoma brucei | |
5170 | - |
D-glyceraldehyde 3-phosphate | pH 7.4, 25°C, mutant enzyme C14A | Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
2-Phosphoglycolate | wild-type enzyme | Trypanosoma brucei | |
0.076 | - |
2-Phosphoglycolate | mutant enzyme C14F | Trypanosoma brucei |