Literature summary for 5.3.1.1 extracted from

Bell, G.S.; Russell, R.J.; Kohlhoff, M.; Hensel, R.; Danson, M.J.; Hough, D.W.; Taylor, G.L.
Preliminary crystallographic studies of triosephosphate isomerase (TIM) from the hyperthermophilic archaeon Pyrococcus woesei (1998), Acta Crystallogr. Sect. D, 54, 1419-1421.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus woesei

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, diffraction quality can only be obtained in the presence of inhibitors, monoclinic crystal form is obtained by cocrystallization with 20 mM 2-carboxyethylphosphonic acid with sodium acetate, pH 4.0, and 7% PEG 4000 as the precipitant buffer. An orthorhombic crystal form is obtained by cocrystallization with 20 mM 2-phosphoglycolic acid with 0.1 M sodium acetate, pH 4.2, and 5% PEG 4000 as the precipitant buffer. Hexagonal crystal forms are obtained under the same conditions with 2-phosphoglycolic acid, but their diffraction shows high disorder	Pyrococcus woesei

Crystallization (Comment)

Organism

hanging drop vapor diffusion method, diffraction quality can only be obtained in the presence of inhibitors, monoclinic crystal form is obtained by cocrystallization with 20 mM 2-carboxyethylphosphonic acid with sodium acetate, pH 4.0, and 7% PEG 4000 as the precipitant buffer. An orthorhombic crystal form is obtained by cocrystallization with 20 mM 2-phosphoglycolic acid with 0.1 M sodium acetate, pH 4.2, and 5% PEG 4000 as the precipitant buffer. Hexagonal crystal forms are obtained under the same conditions with 2-phosphoglycolic acid, but their diffraction shows high disorder

Pyrococcus woesei

Inhibitors

Inhibitors	Comment	Organism	Structure
2-carboxyethylphosphonic acid	-	Pyrococcus woesei
2-phosphoglycolic acid	-	Pyrococcus woesei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	4 * 24000, crystallographic data	Pyrococcus woesei

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus woesei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Pyrococcus woesei

Subunits

Subunits	Comment	Organism
tetramer	4 * 24000, crystallographic data	Pyrococcus woesei

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Release 2023.1 (January 2023)