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Literature summary for 5.2.1.8 extracted from

  • Erben, E.D.; Valguarnera, E.; Nardelli, S.; Chung, J.; Daum, S.; Potenza, M.; Schenkman, S.; Tellez-Inon, M.T.
    Identification of an atypical peptidyl-prolyl cis/trans isomerase from trypanosomatids (2010), Biochim. Biophys. Acta, 1803, 1028-1037.
    View publication on PubMed
Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus Par45 is mainly concentrated in the nucleus Trypanosoma cruzi 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45400
-
 x * 45400, calculated from amino acid sequence Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi B8XP93
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 75 gel filtration Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Trypanosoma cruzi
-
epimastigote
-
 Trypanosoma cruzi
-
trypomastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Par45 does not accelerate the cis/trans interconversion of acidic substrates containing Glu-Pro bonds Trypanosoma cruzi ?
-
 ?
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide 6.9% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide
-
 ?
N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide 100% activity, Par45 shows a strong preference for a substrate with the basic Arg residue preceding Pro Trypanosoma cruzi N-succinyl-Ala-Arg-(trans)-Pro-Phe-4-nitroanilide
-
 ?
N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide 7.6% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide
-
 ?
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide 26.7% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide
-
 ?
N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide 4.3% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide Trypanosoma cruzi N-succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide
-
 ?

Subunits

Subunits Comment Organism
? x * 45400, calculated from amino acid sequence Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
Par45
-
 Trypanosoma cruzi
peptidyl-prolyl cis/trans isomerase
-
 Trypanosoma cruzi
PPIase
-
 Trypanosoma cruzi

General Information

General Information Comment Organism
physiological function Par45 is a phosphorylation-independent parvulin required for normal cell proliferation in a unicellular eukaryotic cell Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2
-
 N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
6.7
-
 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
7.4
-
 N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
25.9
-
 N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi
97.1
-
 N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 Trypanosoma cruzi