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Literature summary for 5.2.1.8 extracted from

  • Boldbaatar, D.; Kilonzo, R.M.; Battur, B.; Umemiya, R.; Liao, M.; Tanaka, T.; Xuan, X.; Fujisaki, K.
    Identification of two forms of cyclophilin from the hard tick Haemaphysalis longicornis (2008), Process Biochem., 43, 615-625.
No PubMed abstract available
Search for more literature for 5.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tag Haemaphysalis longicornis

Protein Variants

Protein Variants Comment Organism
additional information silencing of isoform cyclophilin A by RNAi leads to a significant reduction in the body weight of engorged ticks and their failure to lay eggs Haemaphysalis longicornis
additional information silencing of isoform cyclophilin B by RNAi does not lead to detectable phenotypic changes Haemaphysalis longicornis

Inhibitors

Inhibitors Comment Organism Structure
cyclosporin A
-
 Haemaphysalis longicornis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide kcat/Km ratio is 14.1 per mM and s Haemaphysalis longicornis
additional information
-
 succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide kcat/Km ratio is 21 per mM and s Haemaphysalis longicornis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20100
-
 x * 20100, calculated, x * 24000, SDS-PAGE Haemaphysalis longicornis
23500
-
 x * 23500, calculated for mature protein, x * 23500, SDS-PAGE Haemaphysalis longicornis
24000
-
 x * 20100, calculated, x * 24000, SDS-PAGE Haemaphysalis longicornis

Organism

Organism UniProt Comment Textmining
Haemaphysalis longicornis B2ZWT4 isoform cyclophilin A
-
Haemaphysalis longicornis B2ZWT5 isoform cyclophilin B
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative signal peptide cleavage site between residues S19 and A20 Haemaphysalis longicornis

Source Tissue

Source Tissue Comment Organism Textmining
epidermis
-
 Haemaphysalis longicornis
-
additional information mRNA expression level is homogeneous in different tissues Haemaphysalis longicornis
-
ovary
-
 Haemaphysalis longicornis
-
salivary gland
-
 Haemaphysalis longicornis
-
synganglion
-
 Haemaphysalis longicornis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide
-
 Haemaphysalis longicornis succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide
-
 ?

Subunits

Subunits Comment Organism
? x * 20100, calculated, x * 24000, SDS-PAGE Haemaphysalis longicornis
? x * 23500, calculated for mature protein, x * 23500, SDS-PAGE Haemaphysalis longicornis

pI Value

Organism Comment pI Value Maximum pI Value
Haemaphysalis longicornis calculated
-
 8.3
Haemaphysalis longicornis calculated
-
 9.1