Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
General Stability | Organism |
---|---|
enzyme is active after 10 freeze-thawing cycles | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight inhibition | Agrobacterium tumefaciens | |
Cu2+ | strong inhibition | Agrobacterium tumefaciens | |
Hg2+ | strong inhibition | Agrobacterium tumefaciens | |
additional information | not inhibitory: EDTA, Mn2+, dithiothreitol | Agrobacterium tumefaciens | |
Ni2+ | slight inhibition | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
L-isobutylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
4.26 | - |
D-ethylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
4.45 | - |
L-ethylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
4.47 | - |
D-5-methylthioethylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
4.58 | - |
D-isobutylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
4.71 | - |
D-benzylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
5.41 | - |
L-5-methylthioethylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens | |
5.56 | - |
L-benzylhydantoin | pH 7.5, 40°C | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Agrobacterium tumefaciens |
100000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Agrobacterium tumefaciens |
Storage Stability | Organism |
---|---|
-20°C, pH 8.0, 20% glycerol, stable for at least 6 months | Agrobacterium tumefaciens |
4°C, pH 8.0, stable for 8 weeks | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5-methylthioethylhydantoin | - |
Agrobacterium tumefaciens | D-5-methylthioethylhydantoin | - |
r | |
L-5-methylthioethylhydantoin | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-5-methylthioethylhydantoin | - |
r | |
L-benzylhydantoin | slow racemization | Agrobacterium tumefaciens | D-benzylhydantoin | - |
r | |
L-benzylhydantoin | slow racemization | Agrobacterium tumefaciens C58 / ATCC 33970 | D-benzylhydantoin | - |
r | |
L-ethylhydantoin | - |
Agrobacterium tumefaciens | D-ethylhydantoin | - |
r | |
L-ethylhydantoin | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-ethylhydantoin | - |
r | |
L-isobutylhydantoin | - |
Agrobacterium tumefaciens | D-isobutylhydantoin | - |
r | |
L-isobutylhydantoin | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | D-isobutylhydantoin | - |
r | |
additional information | preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings | Agrobacterium tumefaciens | ? | - |
? | |
additional information | preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings | Agrobacterium tumefaciens C58 / ATCC 33970 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Agrobacterium tumefaciens |