Application | Comment | Organism |
---|---|---|
biotechnology | coupled bioconversion for preparation of N-acetyl-D-neuraminic acid using immobilized N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid lyase, a two-step enzymatic system involving immobilized both enzymes is used for the conversion of GlcNAc to NeuAc in a single reactor, optimum ratio is 3-6.25 U/ml of N-acetyl-D-glucosamine-2-epimerase and 12.5-25 U/ml of N-acetyl-D-neuraminic acid lyase | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | Sus scrofa | - |
N-acetyl-D-glucosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P17560 | - |
- |
Purification (Comment) | Organism |
---|---|
by FP-based affinity chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Escherichia coli cells harboring the recombinant plasmid are grown at 37°C in TB medium and induced by adding lactose to a final concentration of 1% lactose, samples are taken every 2 h, and the highest activity is 0.825 U/ml after fermentation for 22 h, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) | Sus scrofa |
0.07818 | - |
AGE, immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 30% of the free enzyme, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) | Sus scrofa |
0.49 | - |
specific activity in crude extract after expression in Escherichia coli cells, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) | Sus scrofa |
29.13 | - |
recombinant enzyme after purification, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | - |
Sus scrofa | N-acetyl-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGE | - |
Sus scrofa |
N-acetyl-D-glucosamine-2-epimerase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |