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Literature summary for 5.1.3.2 extracted from
- Characterization of UDP-glucose 4-epimerase from Pyrococcus horikoshii: regeneration of UDP to produce UDP-galactose using two-enzyme system with trehalose (2012), Biores. Technol., 110, 423-429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, 95% loss of activity | Pyrococcus horikoshii |  |
| Dimethylsulfoxide | 10%, 26% inhibition | Pyrococcus horikoshii | |
| methanol | 10%, 26% inhibition | Pyrococcus horikoshii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii | |
| 2.24 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, activity is increased by 30% | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Pyrococcus horikoshii |
| 70000 | - |
gel filtration | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O73960 | - |
- |
| Pyrococcus horikoshii OT-3 | O73960 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactose | - |
Pyrococcus horikoshii | UDP-alpha-D-glucose | - |
? | |
| UDP-alpha-D-galactose | - |
Pyrococcus horikoshii OT-3 | UDP-alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GalE | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, stable up to | Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii | |
| 1.1 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pyrococcus horikoshii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 80% of maximal activit, pH 9.0: about 50% of maximal activity | Pyrococcus horikoshii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | highly stable | Pyrococcus horikoshii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
UDP-alpha-D-glucose | pH 6.5, 60°C | Pyrococcus horikoshii | |
| 4.34 | - |
UDP-alpha-D-galactose | pH 6.5, 60°C | Pyrococcus horikoshii | |
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