Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pyrobaculum calidifontis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 29% (v/v) 2-ethoxyethanol and 0.1 M cacodylate buffer (pH 6.6), at 20°C | Pyrobaculum calidifontis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a GalE loop deletion mutant (mut1), in which residues 32-43 (NLSSGRREFVNP) of the NAD-binding loop are replaced with residues 33-40 (IVQRDTGG) of the corresponding loop from Thermoplasma volcanium binds NAD+ in a loose, reversible manner | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
UDP-galactose | in 100 mM MES-NaOH (pH 5.5), at 70°C | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Pyrobaculum calidifontis |
51000 | - |
gel filtration | Pyrobaculum calidifontis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose | Pyrobaculum calidifontis | - |
UDP-alpha-D-galactose | - |
? | |
UDP-galactose | Pyrobaculum calidifontis | - |
UDP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MUJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography and Superdex 200 gel filtration | Pyrobaculum calidifontis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose | - |
Pyrobaculum calidifontis | UDP-alpha-D-galactose | - |
? | |
UDP-galactose | - |
Pyrobaculum calidifontis | UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32000, SDS-PAGE | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
GalE | - |
Pyrobaculum calidifontis |
UDP-galactose-4-epimerase | - |
Pyrobaculum calidifontis |
UGE | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
UDP-galactose | in 100 mM MES-NaOH (pH 5.5), at 70°C | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrobaculum calidifontis |