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Literature summary for 5.1.3.2 extracted from

  • Wasilenko, J.; Fridovich-Keil, J.L.
    Relationship between UDP-galactose 4-epimerase activity and galactose sensitivity in yeast (2006), J. Biol. Chem., 281, 8443-8449.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
To explore the quantitative relationship between GALE activity and galactose metabolism and sensitivity in yeast, a strain is generated in which the expression of GAL10, encoding GALE, was regulated by doxycycline. In brief, a doxycycline-repressible promoter is introduced just upstream of the GAL10 coding sequence in a strain (JFy4763) that was otherwise wild type for all Leloir pathway enzymes and that was engineered to express the appropriate doxycycline-responsive transcriptional activator and repressor protein moieties. Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose Saccharomyces cerevisiae Leloir pathway of galactose metabolism UDP-glucose
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
GALE activity evaluated in samples by monitoring the conversion of UDP-gal to UDP-glc. Assays stopped by the addition of 237.5 microliter of ice-cold water followed immediately by filtration through 0.2-micrometer nylon micro-spin columns (Corning) to remove proteins and particulates before high performance liquid chromatography analysis. Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose Leloir pathway of galactose metabolism Saccharomyces cerevisiae UDP-glucose
-
r

Synonyms

Synonyms Comment Organism
GAL10
-
Saccharomyces cerevisiae
GalE
-
Saccharomyces cerevisiae
UDP-galactose 4'-epimerase
-
Saccharomyces cerevisiae