Cloned (Comment) | Organism |
---|---|
gene dnmU, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli | Streptomyces peucetius |
gene rmbC, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli | Streptomyces peucetius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of rmbC-defective mutant | Streptomyces peucetius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Streptomyces peucetius | - |
dTDP-4-dehydro-beta-L-rhamnose | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | Streptomyces peucetius ATCC 27952 | - |
dTDP-4-dehydro-beta-L-rhamnose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | C5J048 | gene rmbC | - |
Streptomyces peucetius | O33707 | gene dnmU | - |
Streptomyces peucetius ATCC 27952 | C5J048 | gene rmbC | - |
Streptomyces peucetius ATCC 27952 | O33707 | gene dnmU | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces peucetius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | - |
Streptomyces peucetius | dTDP-4-dehydro-beta-L-rhamnose | - |
? | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucose | - |
Streptomyces peucetius ATCC 27952 | dTDP-4-dehydro-beta-L-rhamnose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase | - |
Streptomyces peucetius |
DnmU | - |
Streptomyces peucetius |
dTDP-4-keto-6-deoxyglucose 3,5-epimerase | - |
Streptomyces peucetius |
RmbC | - |
Streptomyces peucetius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces peucetius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces peucetius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces peucetius | |
NADH | - |
Streptomyces peucetius |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of rmbC results in fragmented mycelia that quickly convert into gray pigmented spores | Streptomyces peucetius |
metabolism | the enzyme from the doxorubicin biosynthesis gene cluster is involved in the biosynthesis of dTDP-L-daunosamine, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene rmbC. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other | Streptomyces peucetius |
metabolism | the enzyme is involved in the biosynthesis of dTDP-L-rhamnose, a precursor of cell wall biosynthesis, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene dnmU. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other | Streptomyces peucetius |