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Literature summary for 5.1.3.1 extracted from
- Purification, properties and in situ localization of the amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase from spinach chloroplasts (1989), Eur. J. Biochem., 252, 237-244.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
D-ribulose 5-phosphate | - |
Spinacia oleracea |  |
| 0.25 | - |
D-ribulose 5-phosphate | pH 7.5 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | stroma, 90% of the total epimerase is associated with thylakoid membranes | Spinacia oleracea | 9507 | - |
| chloroplast | stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
8 * 23000, SDS-PAGE | Spinacia oleracea |
| 180000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 5-phosphate | - |
Spinacia oleracea | D-Xylulose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 23000, SDS-PAGE | Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.138 | - |
D-ribulose 5-phosphate | - |
Spinacia oleracea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Spinacia oleracea |
| 8.3 | - |
reaction with D-ribulose 5-phosphate | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 90% of maximal activity, pH 9.0: 95% of maximal activity | Spinacia oleracea |
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Release 2023.1 (January 2023)
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